Rabbit anti-Human MLF1IP Polyclonal Antibody | anti-MLF1IP antibody

MLF1IP, CT (MLF1IP, CENPU, ICEN24, KLIP1, PBIP1, Centromere protein U, Interphase centromere complex protein 24, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, Polo-box-interacting protein 1, centromere protein of 50kD) (PE)

Cat. Num. AAA6321074

• Gene Names: CENPU; KLIP1; PBIP1; CENP50; MLF1IP; CENPU50
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MLF1IP; Polyclonal Antibody; CT (MLF1IP; CENPU; ICEN24; KLIP1; PBIP1; Centromere protein U; Interphase centromere complex protein 24; KSHV latent nuclear antigen-interacting protein 1; MLF1-interacting protein; Polo-box-interacting protein 1; centromere protein of 50kD) (PE); centromere protein of 50kD; anti-MLF1IP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MLF1IP antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MLF1IP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-319 amino acids from the C-terminal region of human MLF1IP.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MLF1IP Antibody (C-term) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the MLF1IP antibody detected the MLF1IP protein (arrow).)

Related Product Information for anti-MLF1IP antibody

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).

Product Categories/Family for anti-MLF1IP antibody

Antibodies; Centromeres

References

Suzuki, H., et al. Anticancer Res. 27 (3B), 1423-1430 (2007): Kang, Y.H., et al. Mol. Cell 24(3):409-422(2006). Izuta, H., et al. Genes Cells 11(6):673-684(2006). Okada, M., et al. Nat. Cell Biol. 8(5):446-457(2006). Foltz, D.R., et al. Nat. Cell Biol. 8(5):458-469(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 38016935
• NCBI GeneID: 79682
• NCBI Accession #: NP_078905.2
• NCBI GenBank Nucleotide #: NM_024629.3
• UniProt Accession #: Q71F23; Q09GN2; Q32Q71; Q9H5G1; A2RRD9
• Molecular Weight: 38,102 Da
• NCBI Official Full Name: centromere protein U
• NCBI Official Synonym Full Names: centromere protein U
• NCBI Official Symbol: CENPU
• NCBI Official Synonym Symbols: KLIP1; PBIP1; CENP50; MLF1IP; CENPU50
• NCBI Protein Information: centromere protein U; KSHV latent nuclear antigen interacting protein 1; KSHV latent nuclear antigen-interacting protein 1; MLF1 interacting protein; MLF1-interacting protein; centromere protein of 50 kDa; interphase centromere complex protein 24; polo-bo
• UniProt Protein Name: Centromere protein U
• UniProt Gene Name: CENPU
• UniProt Synonym Gene Names: ICEN24; KLIP1; MLF1IP; PBIP1; CENP-U; CENP-50
• UniProt Entry Name: CENPU_HUMAN

NCBI Description

The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). MLF1IP, or CENPU, is an additional factor required for centromere assembly (Foltz et al., 2006 [PubMed 16622419]).[supplied by OMIM, Mar 2008]

Uniprot Description

CENPU: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: nucleoplasm; microtubule organizing center; nucleus; cytosol

Biological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; chordate embryonic development; transcription, DNA-dependent; viral reproduction; regulation of transcription, DNA-dependent; mitotic cell cycle