Rabbit anti-Human, Mouse MITF Polyclonal Antibody | anti-MITF antibody

MITF (Phospho-Ser180/73) Antibody

Cat. Num. AAA9508640

• Gene Names: MITF; MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: MITF; Polyclonal Antibody; MITF (Phospho-Ser180/73) Antibody; MITF M1; Microphthalmia-associated transcription factor; anti-MITF antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: MITF (Phospho-Ser180/Ser73) Antibody detects endogenous levels of MITF only when phosphorylated at Ser180/Ser73.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 468

• Applicable Applications for anti-MITF antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:S180; M:S180
• Immunogen: The antiserum was produced against synthesized phospho-peptide derived from human MITF around the phosphorylation site of Ser180/Ser73.
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MITF (Phospho-Ser180/73) Antibody)

Western Blot (WB)

(Western blot analysis of lysates from COS7 cells, using MITF (Phospho-Ser180/73) Antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4557755
• NCBI GeneID: 4286
• NCBI Accession #: NP_000239.1
• NCBI GenBank Nucleotide #: NM_000248.3
• UniProt Accession #: O75030; Q14841; Q9P2V0; Q9P2V1; Q9P2V2; Q9P2Y8; B4DJL2; D3K197; E9PFN0
• Molecular Weight: 58 kDa
• NCBI Official Full Name: microphthalmia-associated transcription factor isoform 4
• NCBI Official Synonym Full Names: melanogenesis associated transcription factor
• NCBI Official Symbol: MITF
• NCBI Official Synonym Symbols: MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
• NCBI Protein Information: microphthalmia-associated transcription factor
• UniProt Protein Name: Microphthalmia-associated transcription factor
• Protein Family: Microphthalmia-associated transcription factor
• UniProt Gene Name: MITF
• UniProt Synonym Gene Names: BHLHE32; bHLHe32

NCBI Description

The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]

Uniprot Description

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.