Rabbit anti-Human Mimitin Polyclonal Antibody | anti-NDUFAF2 antibody

Mimitin Antibody

Cat. Num. AAA150314

• Gene Names: NDUFAF2; MMTN; B17.2L; mimitin; NDUFA12L
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: Mimitin Antibody is affinity chromatography purified via peptide column.
• Synonyms: Mimitin; Polyclonal Antibody; Mimitin Antibody; MMTN; B17.2L; mimitin; NDUFA12L; mitochondrial; Myc-induced mitochondrial protein; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex; assembly factor 2; anti-NDUFAF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Mimitin Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 169

• Applicable Applications for anti-NDUFAF2 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Mimitin antibody can be used for detection of Mimitin by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunoflourescence starting at 20 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Mimitin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Mimitin.
• Buffer: Mimitin Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Mimitin antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Mimitin in Raji cell lysate with Mimitin antibody at (A) 1 and (B) 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Mimitin in Raji cells with Mimitin antibody at 20 μg/mL.)

Related Product Information for anti-NDUFAF2 antibody

Mimitin Antibody: Mimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-κB-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I.

NCBI and Uniprot Product Information

• NCBI GI #: 29789409
• NCBI GeneID: 91942
• NCBI Accession #: NP_777549
• NCBI GenBank Nucleotide #: NM_174889.4
• UniProt Accession #: Q8N183; A8K5I1
• Molecular Weight: 19,856 Da
• NCBI Official Full Name: mimitin, mitochondrial
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) complex I, assembly factor 2
• NCBI Official Symbol: NDUFAF2
• NCBI Official Synonym Symbols: MMTN; B17.2L; mimitin; NDUFA12L
• NCBI Protein Information: mimitin, mitochondrial; NDUFA12-like protein; Myc-induced mitochondrial protein; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2
• UniProt Protein Name: Mimitin, mitochondrial
• Protein Family: Mimitin
• UniProt Gene Name: NDUFAF2
• UniProt Synonym Gene Names: NDUFA12L; B17.2L; MMTN
• UniProt Entry Name: MIMIT_HUMAN

NCBI Description

NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene cause progressive encephalopathy resulting from mitochondrial complex I deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

NDUFAF2: Acts as a molecular chaperone for mitochondrial complex I assembly. Defects in NDUFAF2 are a cause of mitochondrial complex I deficiency (MT-C1D). A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease. Belongs to the complex I NDUFA12 subunit family.

Chromosomal Location of Human Ortholog: 5q12.1

Cellular Component: mitochondrion

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Disease: Leigh Syndrome; Mitochondrial Complex I Deficiency