Western Blot (WB)
(MIER2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MIER2 antibody detected the MIER2 protein (arrow).)
Rabbit anti-Human MIER2 Polyclonal Antibody | anti-MIER2 antibody
MIER2 Antibody (N-term)
Gene Names
MIER2; Mi-er2; KIAA1193
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MIER2; Polyclonal Antibody; MIER2 Antibody (N-term); Mesoderm induction early response protein 2; Mi-er2; KIAA1193; anti-MIER2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MIER2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-108 amino acids from the N-terminal region of human MIER2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
79-108
Sequence Length
545
Applicable Applications for anti-MIER2 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(MIER2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MIER2 antibody detected the MIER2 protein (arrow).)
Western Blot (WB)
(MIER2 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MIER2 antibody detected the MIER2 protein (arrow).)
Flow Cytometry (FC/FACS)
(MIER2 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(MIER2 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-MIER2 antibody
Hydrolyzes ADP-ribose, IDP-ribose, CDP-glycerol, CDP-choline and CDP-ethanolamine, but not other non-reducing ADP-sugars or CDP-glucose. May be involved in immune cell signaling as suggested by the second-messenger role of ADP-ribose, which activates TRPM2 as a mediator of oxidative/nitrosative stress (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59944
NCBI Official Full Name
mesoderm induction early response protein 2
NCBI Official Synonym Full Names
mesoderm induction early response 1, family member 2
NCBI Official Symbol
MIER2
NCBI Official Synonym Symbols
Mi-er2; KIAA1193
NCBI Protein Information
mesoderm induction early response protein 2
UniProt Protein Name
Mesoderm induction early response protein 2
Protein Family
UniProt Gene Name
MIER2
UniProt Synonym Gene Names
KIAA1193; Mi-er2
UniProt Entry Name
MIER2_HUMAN
Uniprot Description
MIER2: Transcriptional repressor.
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleus
Molecular Function: DNA binding; chromatin binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
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Product Notes
The MIER2 mier2 (Catalog #AAA9204981) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 79-108. The MIER2 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIER2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the MIER2 mier2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIER2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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