Testing Data
(The Mib Pab is used at 1:1000 to detect DYKDDDDK-tagged Mib in 293T cell lysate. Data courtesy of Dr. Olga Ossipova, Mt. Sinai School of Medicine.)
Rabbit anti-Human Mib1 Polyclonal Antibody | anti-Mib1 antibody
Mib1, NT (E3 Ubiquitin-protein Ligase MIB1, Mind Bomb Homolog 1, DAPK-interacting Protein 1, DIP-1, Zinc Finger ZZ Type With Ankyrin Repeat Domain Protein 2, MIB1, DIP1, KIAA1323, ZZANK2) (AP)
ELISA: 1:1000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Testing Data
(The Mib Pab is used at 1:1000 to detect DYKDDDDK-tagged Mib in 293T cell lysate. Data courtesy of Dr. Olga Ossipova, Mt. Sinai School of Medicine.)
Testing Data
(The Mib Pab is used at 1:1000 to detect DYKDDDDK-tagged Mib in 293T cell lysate. Data courtesy of Dr. Olga Ossipova, Mt. Sinai School of Medicine.)
Immunofluorescence (IF)
(Immunofluorescent staining of Hela cells incubated with MIB Antibody (N-term) at a dilution of 1:20. Data courtesy of Dr. Vyacheslav Akimov, University of Southern Denmark.)
Immunofluorescence (IF)
(Immunofluorescent staining of Hela cells incubated with MIB Antibody (N-term) at a dilution of 1:20. Data courtesy of Dr. Vyacheslav Akimov, University of Southern Denmark.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
NCBI and Uniprot Product Information
Observed: 103 kDa
NCBI Description
This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). [provided by RefSeq, Jun 2013]
Uniprot Description
MIB1: E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF- induced apoptosis. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation. Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
Protein type: Ubiquitin conjugating system; EC 6.3.2.-; EC 6.3.2.19; Ubiquitin ligase; Ligase
Chromosomal Location of Human Ortholog: 18q11.2
Cellular Component: centrosome; postsynaptic density; cytoplasm; plasma membrane; cytoplasmic vesicle; cytosol
Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: blood vessel development; somitogenesis; negative regulation of neuron differentiation; Notch signaling pathway; in utero embryonic development; protein ubiquitination; positive regulation of endocytosis; endocytosis; heart looping; neural tube formation
Disease: Left Ventricular Noncompaction 7
Research Articles on Mib1
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Product Notes
The Mib1 mib1 (Catalog #AAA6487975) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mib1, NT (E3 Ubiquitin-protein Ligase MIB1, Mind Bomb Homolog 1, DAPK-interacting Protein 1, DIP-1, Zinc Finger ZZ Type With Ankyrin Repeat Domain Protein 2, MIB1, DIP1, KIAA1323, ZZANK2) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mib1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Mib1 mib1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mib1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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