Rabbit anti-Human MHC class I Polyclonal Antibody | anti-MHC antibody

MHC class I Polyclonal Antibody

Cat. Num. AAA3013039

• Gene Names: HLA-A; HLAA
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: MHC class I; Polyclonal Antibody; MHC class I Polyclonal Antibody; HLA-A; HLA-B; A 28; A 9; Antigen presenting molecule; Aw 24; Aw 68; CLASS I HISTOCOMPATIBILITY ANTIGEN; H2 K1; H2K; HLA A; HLA class I histocompatibility antigen A 1 alpha chain; HLA class I histocompatibility antigen A 11 alpha chain; HLA class I histocompatibility antigen A 2 alpha chain; HLA class I histocompatibility antigen A 24 alpha chain; HLA class I histocompatibility antigen A 3 alpha chain; HLA class I histocompatibility antigen A 30 alpha chain; HLA class I histocompatibility antigen A 32 alpha chain; HLA class I histocompatibility antigen A 68 alpha chain; HLAA; Leukocyte antigen class I A; Major histocompatibility complex class I A; MHC class I antigen A 1; MHC class I antigen A*11; MHC class I antigen A*2; MHC class I antigen A*24; MHC class I antigen A*3; MHC class I antigen A*30; MHC class I antigen A*32; MHC class I antigen A*68; MHC class I antigen HLA A heavy chain; MHC class I heavy chain H2 K; anti-MHC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MHC class I polyclonal antibody detects endogenous levels of MHC class I protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 365

• Applicable Applications for anti-MHC antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1,000-1:5,000; ICC: 1:100-1:500; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of MHC class I on different lysates using anti-MHC class I antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: THP-1)

Immunocytochemistry (ICC)

(ICC staining MHC class I in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-MHC antibody

Major histocompatibility complex (MHC) molecules, also designated human leukocyte antigen (HLA) molecules, are cell-surface receptors that bind foreign peptides and present them to T lymphocytes. MHC class I molecules consist of two polypeptide chains, an a or heavy chain, and beta-2-Microglobulin, a non-covalently associated protein. Cytotoxic T lymphocytes bind antigenic peptides presented by MHC class I molecules. Antigens that bind to MHC class I molecules are typically 8-10 residues in length and are stabilized in a peptide binding groove. MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an a and b chain. Helper T lymphocytes bind antigenic peptides presented by MHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.

NCBI and Uniprot Product Information

• NCBI GI #: 337752170
• NCBI GeneID: 3105
• NCBI Accession #: NP_001229687.1
• NCBI GenBank Nucleotide #: NP_001229687.1
• UniProt Accession #: P30443; P01889; P01891; P01892; P13746; P16188; P30450; P04439
• Molecular Weight: 41kDa
• NCBI Official Full Name: HLA class I histocompatibility antigen, A alpha chain A*01:01:01:01
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, A
• NCBI Official Symbol: HLA-A
• NCBI Official Synonym Symbols: HLAA
• NCBI Protein Information: HLA class I histocompatibility antigen, A alpha chain
• UniProt Protein Name: HLA class I histocompatibility antigen, A-1 alpha chain
• Protein Family: Myosin
• UniProt Gene Name: HLA-A
• UniProt Synonym Gene Names: HLAA

NCBI Description

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the presentation of foreign antigens to the immune system.