Western Blot (WB)
(Western blot analysis of MGCRACGAP expression in A431 (A), Raji (B), COS7 (C) whole cell lysates.)
Rabbit MGCRACGAP Polyclonal Antibody | anti-RACGAP1 antibody
Anti-MGCRACGAP Antibody
Western Blot (WB)
(Western blot analysis of MGCRACGAP expression in A431 (A), Raji (B), COS7 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of MGCRACGAP expression in A431 (A), Raji (B), COS7 (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of MGCRACGAP staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of MGCRACGAP staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of MGCRACGAP staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of MGCRACGAP staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12. [provided by RefSeq, Feb 2016]
Uniprot Description
MgcRacGAP: a rac GTPase activating protein. Plays an important role in cytokinesis by regulating cortical movement through RhoA. Phosphorylation by Aurora B apparently induces GAP activity toward RhoA.
Protein type: GAPs, Rac/Rho; GAPs
Chromosomal Location of Human Ortholog: 12q13.12
Cellular Component: acrosome; cleavage furrow; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; microtubule; midbody; nucleoplasm; nucleus; spindle midzone
Molecular Function: alpha-tubulin binding; beta-tubulin binding; gamma-tubulin binding; GTPase activator activity; metal ion binding; microtubule binding; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; protein kinase binding
Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; blood coagulation; cytokinesis after mitosis; cytokinesis, contractile ring formation; embryonic development; microtubule-based movement; neuroblast proliferation; positive regulation of cytokinesis; positive regulation of GTPase activity; regulation of attachment of spindle microtubules to kinetochore; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; spermatogenesis; spindle midzone assembly involved in mitosis; sulfate transport
Research Articles on RACGAP1
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Product Notes
The RACGAP1 racgap1 (Catalog #AAA8242110) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MGCRACGAP Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's MGCRACGAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500). Researchers should empirically determine the suitability of the RACGAP1 racgap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGCRACGAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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