Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysate, HepG2 whole cell lysateAll lanes: MFSD4A antibody at 3.38ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 57, 47 KDaObserved band size: 57 KDa)
Rabbit anti-Human MFSD4A Polyclonal Antibody | anti-MFSD4A antibody
MFSD4A Antibody
Gene Names
MFSD4A; MFSD4; UNQ3064
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
MFSD4A; Polyclonal Antibody; MFSD4A Antibody; Major facilitator superfamily domain-containing protein 4A; Major facilitator superfamily domain-containing protein 4; anti-MFSD4A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
514
Applicable Applications for anti-MFSD4A antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:5000
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant Human Major facilitator superfamily domain-containing protein 4A protein (160-220AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysate, HepG2 whole cell lysateAll lanes: MFSD4A antibody at 3.38ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 57, 47 KDaObserved band size: 57 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: A549 whole cell lysate, HepG2 whole cell lysateAll lanes: MFSD4A antibody at 3.38ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 57, 47 KDaObserved band size: 57 KDa)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7110403 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7110403 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Related Product Information for anti-MFSD4A antibody
glucose transmembrane transporter activity
Product Categories/Family for anti-MFSD4A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,849 Da
NCBI Official Full Name
major facilitator superfamily domain-containing protein 4A
NCBI Official Synonym Full Names
major facilitator superfamily domain containing 4A
NCBI Official Symbol
MFSD4A
NCBI Official Synonym Symbols
MFSD4; UNQ3064
NCBI Protein Information
major facilitator superfamily domain-containing protein 4A
UniProt Protein Name
Major facilitator superfamily domain-containing protein 4A
Protein Family
UniProt Gene Name
MFSD4A
Research Articles on MFSD4A
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Product Notes
The MFSD4A mfsd4a (Catalog #AAA7110403) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MFSD4A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MFSD4A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:5000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the MFSD4A mfsd4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MFSD4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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