Western Blot (WB)
(Western blot analysis of extracts from various samples, using MFAP5 Antibody. Lane 1: Rat brain treated with blocking peptide; Lane 2: Rat brain; Lane 3: Mouse brain.)
Rabbit MFAP5 Polyclonal Antibody | anti-MFAP5 antibody
MFAP5 Antibody
Gene Names
MFAP5; AAT9; MP25; MAGP2; MAGP-2; MFAP-5
Reactivity
Human, Mouse, RatPredicted: Pig
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
MFAP5; Polyclonal Antibody; MFAP5 Antibody; AAT9; MAGP 2; MAGP-2; MAGP2; MFAP 5; MFAP-5; MFAP5_HUMAN; Microfibril associated glycoprotein 2; Microfibril-associated glycoprotein 2; microfibrillar associated protein 5; Microfibrillar associated protein 5 precursor; Microfibrillar-associated protein 5; MP25; anti-MFAP5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig
Predicted: Pig
Clonality
Polyclonal
Isotype
IgG
Specificity
MFAP5 Antibody detects endogenous levels of MFAP5.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
173
Applicable Applications for anti-MFAP5 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human MFAP5.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Extracellular Region or Secreted
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from various samples, using MFAP5 Antibody. Lane 1: Rat brain treated with blocking peptide; Lane 2: Rat brain; Lane 3: Mouse brain.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using MFAP5 Antibody. Lane 1: Rat brain treated with blocking peptide; Lane 2: Rat brain; Lane 3: Mouse brain.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, using MFAP5 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, using MFAP5 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-MFAP5 antibody
Description: May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity (By similarity). Component of the elastin-associated microfibrils (PubMed:8557636).
Post Translational Modifications: Forms intermolecular disulfide bonds either with other MAGP-2 molecules or with other components of the microfibrils. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. O-glycan heterogeneity at Thr-54: HexHexNAc (major) and HexHexNAc + sulfate (minor).
Subunit Structure: Interacts with TGFB2. Interacts with BMP2. Interacts with FBN1 (via N-terminal domain) and FBN2.
Similarity: Belongs to the MFAP family.
Post Translational Modifications: Forms intermolecular disulfide bonds either with other MAGP-2 molecules or with other components of the microfibrils. N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. O-glycan heterogeneity at Thr-54: HexHexNAc (major) and HexHexNAc + sulfate (minor).
Subunit Structure: Interacts with TGFB2. Interacts with BMP2. Interacts with FBN1 (via N-terminal domain) and FBN2.
Similarity: Belongs to the MFAP family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 20 kDa
Predicted: 20 kDa
Predicted: 20 kDa
NCBI Official Full Name
microfibrillar-associated protein 5 isoform a
NCBI Official Synonym Full Names
microfibril associated protein 5
NCBI Official Symbol
MFAP5
NCBI Official Synonym Symbols
AAT9; MP25; MAGP2; MAGP-2; MFAP-5
NCBI Protein Information
microfibrillar-associated protein 5
UniProt Protein Name
Microfibrillar-associated protein 5
Protein Family
UniProt Gene Name
MFAP5
UniProt Synonym Gene Names
MAGP2; MFAP-5; MAGP-2
UniProt Entry Name
MFAP5_HUMAN
NCBI Description
This gene encodes a 25-kD microfibril-associated glycoprotein which is a component of microfibrils of the extracellular matrix. The encoded protein promotes attachment of cells to microfibrils via alpha-V-beta-3 integrin. Deficiency of this gene in mice results in neutropenia. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
Uniprot Description
MFAP5: Component of the elastin-associated microfibrils. Belongs to the MFAP family.
Protein type: Secreted, signal peptide; Secreted; Extracellular matrix
Chromosomal Location of Human Ortholog: 12p13.1-p12.3
Cellular Component: microfibril; extracellular region
Molecular Function: extracellular matrix structural constituent
Biological Process: extracellular matrix organization and biogenesis; fibril organization and biogenesis
Disease: Aortic Aneurysm, Familial Thoracic 9
Research Articles on MFAP5
Similar Products
Product Notes
The MFAP5 mfap5 (Catalog #AAA9611291) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MFAP5 Antibody reacts with Human, Mouse, Rat Predicted: Pig and may cross-react with other species as described in the data sheet. AAA Biotech's MFAP5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MFAP5 mfap5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MFAP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
