Western Blot (WB)
(Western blot analysis of METTL1 Antibody (Center) in Ramos cell line lysates (35ug/lane). METTL1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human METTL1 Polyclonal Antibody | anti-METTL1 antibody
METTL1, ID (METTL1, C12orf1, tRNA (guanine-N(7)-)-methyltransferase, Methyltransferase-like protein 1, tRNA (guanine(46)-N(7))-methyltransferase, tRNA(m7G46)-methyltransferase) (APC)
Gene Names
METTL1; TRM8; TRMT8; C12orf1; YDL201w
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
METTL1; Polyclonal Antibody; ID (METTL1; C12orf1; tRNA (guanine-N(7)-)-methyltransferase; Methyltransferase-like protein 1; tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase) (APC); tRNA(m7G46)-methyltransferase; anti-METTL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-METTL1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
METTL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154~183 amino acids from the Central region of human METTL1.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of METTL1 Antibody (Center) in Ramos cell line lysates (35ug/lane). METTL1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of METTL1 Antibody (Center) in Ramos cell line lysates (35ug/lane). METTL1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-METTL1 antibody
This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation.
Product Categories/Family for anti-METTL1 antibody
References
Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010). Nat. Genet. 41(7):824-828(2009). Cartlidge, R.A., et al. EMBO J. 24(9):1696-1705(2005). Alexandrov, A., et al. RNA 8(10):1253-1266(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,423 Da
NCBI Official Full Name
tRNA (guanine-N(7)-)-methyltransferase isoform a
NCBI Official Synonym Full Names
methyltransferase like 1
NCBI Official Symbol
METTL1
NCBI Official Synonym Symbols
TRM8; TRMT8; C12orf1; YDL201w
NCBI Protein Information
tRNA (guanine-N(7)-)-methyltransferase; tRNA (guanine-N(7)-)-methyltransferase; D1075-like gene product; METTL1; methyltransferase-like 1; methyltransferase-like protein 1; tRNA (guanine(46)-N(7))-methyltransferase; tRNA(m7G46)-methyltransferase
UniProt Protein Name
tRNA (guanine-N(7)-)-methyltransferase
UniProt Gene Name
METTL1
UniProt Entry Name
TRMB_HUMAN
NCBI Description
This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq, Jul 2008]
Uniprot Description
METTL1: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. Belongs to the methyltransferase superfamily. TrmB family.
Protein type: Methyltransferase; EC 2.1.1.33; RNA processing
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; tRNA (guanine-N7-)-methyltransferase activity; tRNA binding
Biological Process: tRNA modification; tRNA methylation
Research Articles on METTL1
Similar Products
Product Notes
The METTL1 mettl1 (Catalog #AAA6320372) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The METTL1, ID (METTL1, C12orf1, tRNA (guanine-N(7)-)-methyltransferase, Methyltransferase-like protein 1, tRNA (guanine(46)-N(7))-methyltransferase, tRNA(m7G46)-methyltransferase) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's METTL1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the METTL1 mettl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "METTL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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