Rabbit anti-Human Methylene Tetrahydrofolate Dehydrogenase 2 Polyclonal Antibody | anti-MTHFD2 antibody

Methylene Tetrahydrofolate Dehydrogenase 2, CT (MTHFD2, Bifunctional Methylenetetrahydrofolate Dehydrogenase/cyclohydrolase Mitochondrial, NADP+ Dependent Methylenetetrahydrofolate Dehydrogenase 2 Methenyltetrahydrofolate Cyclohydrolase, NMDMC) (Biotin)

Cat. Num. AAA6487912

• Gene Names: MTHFD2; NMDMC
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Methylene Tetrahydrofolate Dehydrogenase 2; Polyclonal Antibody; CT (MTHFD2; Bifunctional Methylenetetrahydrofolate Dehydrogenase/cyclohydrolase Mitochondrial; NADP+ Dependent Methylenetetrahydrofolate Dehydrogenase 2 Methenyltetrahydrofolate Cyclohydrolase; NMDMC) (Biotin); Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 2; Methenyltetrahydrofolate Cyclohydrolase; EC=3.5.4.9; NAD-dependent Methylenetetrahydrofolate Dehydrogenase; EC=1.5.1.15; anti-MTHFD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MTHFD2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MTHFD2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MTHFD2 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MTHFD2 antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). MTHFD2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human Colon carcinoma reacted with MTHFD2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(MTHFD2 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MTHFD2 antibody

Antibodies; Enzymes; Dehydrogenase

References

1. Wang, S.S., Leukemia 23(3), 596-602 (2009). 2. Pawelek, P.D., Biochemistry 37(4), 1109-1115 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 94721354
• NCBI GeneID: 10797
• NCBI Accession #: NP_006627
• NCBI GenBank Nucleotide #: NM_006636.3
• UniProt Accession #: P13995; Q53G90; Q53GV5; Q53S36; Q7Z650
• Molecular Weight: 37.2 kDa (344aa) confirmed by MALDI-TOF
• NCBI Official Full Name: bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
• NCBI Official Synonym Full Names: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
• NCBI Official Symbol: MTHFD2
• NCBI Official Synonym Symbols: NMDMC
• NCBI Protein Information: bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
• UniProt Protein Name: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
• Protein Family: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase
• UniProt Gene Name: MTHFD2
• UniProt Synonym Gene Names: NMDMC
• UniProt Entry Name: MTDC_HUMAN

NCBI Description

This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]

Uniprot Description

MTHFD2: a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009]

Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 1.5.1.15; EC 3.5.4.9; Hydrolase; Mitochondrial; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: cytosol; extracellular space; mitochondrial matrix; mitochondrion

Molecular Function: formate-tetrahydrofolate ligase activity; magnesium ion binding; methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NAD+) activity; methylenetetrahydrofolate dehydrogenase (NADP+) activity; phosphate binding

Biological Process: folic acid metabolic process; one-carbon compound metabolic process; tetrahydrofolate metabolic process