Immunohistochemistry (IHC)
(Immunochemical staining of mouse METAP2 in mouse kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Rabbit anti-Mouse methionyl aminopeptidase 2/METAP2 Polyclonal Antibody | anti-METAP2 antibody
Anti-methionyl aminopeptidase 2/METAP2 Antibody, Rabbit Polyclonal
Gene Names
Metap2; p67; Amp2; Mnpep; p67eIF2; AI047573; AL024412; AU014659; 4930584B20Rik; A930035J23Rik
Reactivity
Mouse
Applications
Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
methionyl aminopeptidase 2/METAP2; Polyclonal Antibody; Anti-methionyl aminopeptidase 2/METAP2 Antibody; Rabbit Polyclonal; METAP2/MAP2 Antibody; Rabbit PAb; Antigen Affinity Purified; methionyl aminopeptidase 2; Anti-4930584B20Rik Antibody; Anti-A930035J23Rik Antibody; Anti-AI047573 Antibody; Anti-AL024412 Antibody; Anti-Amp2 Antibody; Anti-AU014659 Antibody; Anti-Mnpep Antibody; Anti-p67 Antibody; Anti-p67eIF2 Antibody; anti-METAP2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Mouse methionyl aminopeptidase 2/METAP2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-METAP2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IP: 1-4uL/mg of lysate
ELISA: 1:5000-1:10,000
IHC-P: 1:500-1:2000
IP: 1-4uL/mg of lysate
Immunogen
Recombinant Mouse METAP2/MAP2 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Mouse METAP2/MAP2 (rM METAP2/MAP2). METAP2/MAP2 specific IgG was purified by Mouse METAP2/MAP2 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of mouse METAP2 in mouse kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of mouse METAP2 in mouse kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Immunoprecipitation (IP)
(Mouse METAP2 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-Mouse METAP2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse METAP2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 72 kDa)
Immunoprecipitation (IP)
(Mouse METAP2 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-Mouse METAP2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-Mouse METAP2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 72 kDa)
Western Blot (WB)
(Anti-METAP2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDaObserved band size:63 kDa)
Western Blot (WB)
(Anti-METAP2 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDaObserved band size:63 kDa)
Related Product Information for anti-METAP2 antibody
METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a protein that belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.
References
Bennett, et al. (1997) EPR Studies on the Mono-and Dicobalt (II)-Substituted Forms of the Aminopeptidase from Aeromonas proteolytica. Insight into the Catalytic Mechanism of Dinuclear Hydrolases. J Am Chem Soc. 119:1923-33.Johansson, et al. (2008) Dicobalt II-II, II-III, and III-III Complexes as Spectroscopic Models for Dicobalt Enzyme Active Sites. Inorg Chem. 47:5079-92.Bradshaw, et al. (2002) Aminopeptidases and angiogenesis. Essays Biochem. 38: 5-78.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,922 Da
NCBI Official Full Name
methionine aminopeptidase 2
NCBI Official Synonym Full Names
methionine aminopeptidase 2
NCBI Official Symbol
Metap2
NCBI Official Synonym Symbols
p67; Amp2; Mnpep; p67eIF2; AI047573; AL024412; AU014659; 4930584B20Rik; A930035J23Rik
NCBI Protein Information
methionine aminopeptidase 2; MAP 2; metAP 2; peptidase M 2; eIF-2-associated p67; initiation factor 2-associated protein (p67); initiation factor 2-associated 67 kDa glycoprotein
UniProt Protein Name
Methionine aminopeptidase 2
UniProt Gene Name
Metap2
UniProt Synonym Gene Names
Mnpep; P67eif2; MAP 2; MetAP 2; p67; p67eIF2
UniProt Entry Name
MAP2_MOUSE
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Product Notes
The METAP2 metap2 (Catalog #AAA8111699) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-methionyl aminopeptidase 2/METAP2 Antibody, Rabbit Polyclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's methionyl aminopeptidase 2/METAP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IHC-P: 1:500-1:2000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the METAP2 metap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "methionyl aminopeptidase 2/METAP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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