Rabbit anti-Human METAP2 Polyclonal Antibody | anti-METAP2 antibody

METAP2, NT (METAP2, MNPEP, P67EIF2, Methionine aminopeptidase 2, Initiation factor 2-associated 67kD glycoprotein, Peptidase M 2, p67eIF2) (PE)

Cat. Num. AAA6320370

• Gene Names: METAP2; MAP2; MNPEP; p67eIF2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: METAP2; Polyclonal Antibody; NT (METAP2; MNPEP; P67EIF2; Methionine aminopeptidase 2; Initiation factor 2-associated 67kD glycoprotein; Peptidase M 2; p67eIF2) (PE); p67eIF2; anti-METAP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-METAP2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: METAP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human METAP2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(METAP2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the METAP2 antibody detected the METAP2 protein (arrow).)

Western Blot (WB)

(Western blot analysis of METAP2 (arrow) using rabbit polyclonal METAP2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the METAP2 gene.)

Related Product Information for anti-METAP2 antibody

This gene is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2.

Product Categories/Family for anti-METAP2 antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Xiao, Q., et al. Biochemistry 49(26):5588-5599(2010). Wang, X., et al. PLoS ONE 5 (8), E11934 (2010): Selvakumar, P., et al. Mol. Cancer 8, 65 (2009): Warder, S.E., et al. J. Proteome Res. 7(11):4807-4820(2008). Tucker, L.A., et al. Oncogene 27(28):3967-3976(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 5803092
• NCBI GeneID: 10988
• NCBI Accession #: NP_006829.1
• NCBI GenBank Nucleotide #: NM_006838.3
• UniProt Accession #: P50579; Q8NB11; B2RDI8; B4DUX5; G3XA91
• Molecular Weight: 50,510 Da
• NCBI Official Full Name: methionine aminopeptidase 2 isoform 1
• NCBI Official Synonym Full Names: methionyl aminopeptidase 2
• NCBI Official Symbol: METAP2
• NCBI Official Synonym Symbols: MAP2; MNPEP; p67eIF2
• NCBI Protein Information: methionine aminopeptidase 2
• UniProt Protein Name: Methionine aminopeptidase 2
• UniProt Gene Name: METAP2
• UniProt Synonym Gene Names: MNPEP; P67EIF2; MAP 2

NCBI Description

The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.