Rabbit anti-Human METAP1 Polyclonal Antibody | anti-METAP1 antibody

METAP1, ID (METAP1, KIAA0094, Methionine aminopeptidase 1, Peptidase M 1) (Azide free) (HRP)

Cat. Num. AAA6320353

• Gene Names: METAP1; MAP1A; MetAP1A
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: METAP1; Polyclonal Antibody; ID (METAP1; KIAA0094; Methionine aminopeptidase 1; Peptidase M 1) (Azide free) (HRP); Peptidase M 1; anti-METAP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-METAP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: METAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-216 amino acids from the Central region of human METAP1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(METAP1 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the METAP1 antibody detected the METAP1 protein (arrow).)

Related Product Information for anti-METAP1 antibody

METAP1 removes the amino-terminal methionine from nascent proteins. Required for normal progression through the cell cycle.

Product Categories/Family for anti-METAP1 antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Xiao, Q., et al. Biochemistry 49(26):5588-5599(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009). Hu, X.V., et al. Biochemistry 46(44):12833-12843(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 164420681
• NCBI GeneID: 23173
• NCBI Accession #: NP_055958.2
• NCBI GenBank Nucleotide #: NM_015143.2
• UniProt Accession #: P53582; B4E2E6
• Molecular Weight: 43,215 Da
• NCBI Official Full Name: methionine aminopeptidase 1
• NCBI Official Synonym Full Names: methionyl aminopeptidase 1
• NCBI Official Symbol: METAP1
• NCBI Official Synonym Symbols: MAP1A; MetAP1A
• NCBI Protein Information: methionine aminopeptidase 1; MAP 1; metAP 1; peptidase M 1
• UniProt Protein Name: Methionine aminopeptidase 1
• Protein Family: Methionine aminopeptidase
• UniProt Gene Name: METAP1
• UniProt Entry Name: MAP11_HUMAN

Uniprot Description

METAP1: a peptidase that removes the amino-terminal methionine from nascent proteins.

Protein type: EC 3.4.11.18; Protease

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: metalloexopeptidase activity; metal ion binding; aminopeptidase activity

Biological Process: rhodopsin mediated signaling; regulation of translation; phototransduction, visible light; regulation of rhodopsin mediated signaling; peptidyl-methionine modification; proteolysis; N-terminal protein amino acid modification