Western Blot (WB)
(Western blot analysis of Pseudomonas aeruginosa Purified protein showing detection of ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST) Metallothionein protein using Rabbit Anti-Metallothionein Polyclonal Antibody (SPC-717). Lane 1: Molecular Weight Ladder (MW). Lane 2: Blank. Lane 3: Pseudomonas aeruginosa purified protein with GST tag. Load: 25 ug. Block: 1% milk, 2% BSA. Primary Antibody: Rabbit Anti-Metallothionein Polyclonal Antibody (SPC-717) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Mouse Anti-Rabbit IgG: AP at 1:1000 for 60 min at RT. Color Development: Alkaline phosphate activity colorimetric assay. Predicted/Observed Size: ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST). Courtesy of: Michael Lynes and Amy Thees, University of Connecticut.)
Rabbit Metallothionein Polyclonal Antibody | anti-PA2140 antibody
Metallothionein Antibody: ATTO 390
Reactivity
Bacteria (Pseudomonas aeruginosa)
Applications
Western Blot
Purity
Peptide Affinity Purified
Synonyms
Metallothionein; Polyclonal Antibody; Metallothionein Antibody: ATTO 390; Rabbit Anti-Pseudomonas aeruginosa Metallothionein Polyclonal; CES 1 antibody; CES1 antibody; Metallothionein 1A antibody; Metallothionein 1S antibody; Metallothionein 2 antibody; Metallothionein 2A antibody; Metallothionein IA antibody; Metallothionein II antibody; Metallothionein-1A antibody; Metallothionein-IA antibody; Metallothionein2 antibody; MGC32848 antibody; MT 1A antibody; MT 2 antibody; MT 2A antibody; MT IA antibody; MT II antibody; MT-1A antibody; MT-IA antibody; MT1 antibody; MT1A antibody; MT1A_HUMAN antibody; MT1S antibody; MT2 antibody; MT2A antibody; MTC antibody; Probable Metallothionein Antibody; anti-PA2140 antibody
Host
Rabbit
Reactivity
Bacteria (Pseudomonas aeruginosa)
Clonality
Polyclonal
Specificity
Detects ~36 kDa.
Purity/Purification
Peptide Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
79
Applicable Applications for anti-PA2140 antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Target
Metallothionein
Immunogen
Synthetic peptide from the mid-protein of Pseudomonas aeruginosa Metallothionein
Immunogen Species
Pseudomonas aeruginosa
Conjugate
ATTO 390
Storage Buffer
PBS, 50% glycerol, 0.09% sodium azide
PubMed ID
28146278
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of Pseudomonas aeruginosa Purified protein showing detection of ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST) Metallothionein protein using Rabbit Anti-Metallothionein Polyclonal Antibody (SPC-717). Lane 1: Molecular Weight Ladder (MW). Lane 2: Blank. Lane 3: Pseudomonas aeruginosa purified protein with GST tag. Load: 25 ug. Block: 1% milk, 2% BSA. Primary Antibody: Rabbit Anti-Metallothionein Polyclonal Antibody (SPC-717) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Mouse Anti-Rabbit IgG: AP at 1:1000 for 60 min at RT. Color Development: Alkaline phosphate activity colorimetric assay. Predicted/Observed Size: ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST). Courtesy of: Michael Lynes and Amy Thees, University of Connecticut.)
Western Blot (WB)
(Western blot analysis of Pseudomonas aeruginosa Purified protein showing detection of ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST) Metallothionein protein using Rabbit Anti-Metallothionein Polyclonal Antibody (SPC-717). Lane 1: Molecular Weight Ladder (MW). Lane 2: Blank. Lane 3: Pseudomonas aeruginosa purified protein with GST tag. Load: 25 ug. Block: 1% milk, 2% BSA. Primary Antibody: Rabbit Anti-Metallothionein Polyclonal Antibody (SPC-717) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Mouse Anti-Rabbit IgG: AP at 1:1000 for 60 min at RT. Color Development: Alkaline phosphate activity colorimetric assay. Predicted/Observed Size: ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST). Courtesy of: Michael Lynes and Amy Thees, University of Connecticut.)
Product Categories/Family for anti-PA2140 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
8,286 Da
NCBI Official Full Name
metallothionein
NCBI Official Symbol
PA2140
NCBI Protein Information
metallothionein
UniProt Protein Name
Probable metallothionein
Protein Family
UniProt Gene Name
PA2140
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Product Notes
The PA2140 pa2140 (Catalog #AAA8000356) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Metallothionein Antibody: ATTO 390 reacts with Bacteria (Pseudomonas aeruginosa) and may cross-react with other species as described in the data sheet. AAA Biotech's Metallothionein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the PA2140 pa2140 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Metallothionein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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