Western Blot (WB)
(MEP50 pAb tested by Western blot. Whole-cell extract of HeLa cells (20 ug per lane) was probed with MEP50 pAb at a dilution of 1:2,500 dilution (lane 1). In lane 2, the antibody was pre-incubated with 1 uM recombinant MEP50 protein, eliminating the band and demonstrating the specificity of MEP50 pAb for MEP50.)
Rabbit MEP50 Polyclonal Antibody | anti-MEP50 antibody
MEP50 antibody (pAb)
Gene Names
WDR77; p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50
Reactivity
Human, Wide Range Predicted
Applications
Western Blot
Synonyms
MEP50; Polyclonal Antibody; MEP50 antibody (pAb); WDR77; p44; MEP-50; HKMT1069; Nbla10071; p44/Mep50; anti-MEP50 antibody
Host
Rabbit
Reactivity
Human, Wide Range Predicted
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-MEP50 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:1,000-1:2,500 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
WB: 1:1,000-1:2,500 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This MEP50 antibody was raised against full-length human MEP50 with an N-terminal His-Tag.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(MEP50 pAb tested by Western blot. Whole-cell extract of HeLa cells (20 ug per lane) was probed with MEP50 pAb at a dilution of 1:2,500 dilution (lane 1). In lane 2, the antibody was pre-incubated with 1 uM recombinant MEP50 protein, eliminating the band and demonstrating the specificity of MEP50 pAb for MEP50.)
Western Blot (WB)
(MEP50 pAb tested by Western blot. Whole-cell extract of HeLa cells (20 ug per lane) was probed with MEP50 pAb at a dilution of 1:2,500 dilution (lane 1). In lane 2, the antibody was pre-incubated with 1 uM recombinant MEP50 protein, eliminating the band and demonstrating the specificity of MEP50 pAb for MEP50.)
Related Product Information for anti-MEP50 antibody
Short Description: Our MEP50 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples, but it is predicted that it will react with a wide range of sample types.
Background: MEP50 (also designated WDR77) is an important component of the methylosome, a large (20S) protein arginine methyltransferase complex that contains PRMT5. The methylosome functions to modify specific arginines to dimethylarginines in the arginine- and glycine-rich domains of several spliceosomal Sm proteins. This modification targets these proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein (snRNP) core particles. A 50 kDa WD repeat protein MEP50 binds to JBP1 and a subset of Sm proteins and is thought to provide an interface for multiple protein interactions between methylosome proteins. The ability of MEP50 to interact with multiple proteins suggests it is important for targeting the Sm as well as other yet unknown proteins to the methylosome for symmetrical dimethylation. MEP50 specifically interacts with CTD phosphatase FCP1 (CTDP1), a protein that may link the processes of transcriptional elongation and splicing, and with Suz12, a polycomb group protein involved in transcriptional repression.
Background: MEP50 (also designated WDR77) is an important component of the methylosome, a large (20S) protein arginine methyltransferase complex that contains PRMT5. The methylosome functions to modify specific arginines to dimethylarginines in the arginine- and glycine-rich domains of several spliceosomal Sm proteins. This modification targets these proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein (snRNP) core particles. A 50 kDa WD repeat protein MEP50 binds to JBP1 and a subset of Sm proteins and is thought to provide an interface for multiple protein interactions between methylosome proteins. The ability of MEP50 to interact with multiple proteins suggests it is important for targeting the Sm as well as other yet unknown proteins to the methylosome for symmetrical dimethylation. MEP50 specifically interacts with CTD phosphatase FCP1 (CTDP1), a protein that may link the processes of transcriptional elongation and splicing, and with Suz12, a polycomb group protein involved in transcriptional repression.
Product Categories/Family for anti-MEP50 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,651 Da
NCBI Official Full Name
methylosome protein 50
NCBI Official Synonym Full Names
WD repeat domain 77
NCBI Official Symbol
WDR77
NCBI Official Synonym Symbols
p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50
NCBI Protein Information
methylosome protein 50; WD repeat-containing protein 77; androgen receptor cofactor p44
UniProt Protein Name
Methylosome protein 50
UniProt Gene Name
WDR77
UniProt Synonym Gene Names
MEP50; WD45; MEP-50
UniProt Entry Name
MEP50_HUMAN
Uniprot Description
WDR77: a WD40 repeat protein. Component of the methylosome, a 20S complex containing at least ICLN, SKB1 and WDR77. Interacts with SKB1 and with SM proteins. The methylosome may regulate an early step in the assembly of U snRNPs, possibly the transfer of Sm proteins to the SMN-complex. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.
Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 1p13.2
Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleus; cytosol
Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity
Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; spliceosomal snRNP biogenesis; positive regulation of cell proliferation; gene expression
Similar Products
Product Notes
The MEP50 wdr77 (Catalog #AAA388395) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MEP50 antibody (pAb) reacts with Human, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's MEP50 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:1,000-1:2,500 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the MEP50 wdr77 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEP50, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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