Western Blot (WB)
(Western blot analysis of anti-MEN1 Antibody (T594) in Ramos cell line lysates (35ug/lane). MEN1(arrow) was detected using the purified Pab.)
Rabbit MEN1 Polyclonal Antibody | anti-MEN1 antibody
MEN1 Antibody (T594)
Western Blot (WB)
(Western blot analysis of anti-MEN1 Antibody (T594) in Ramos cell line lysates (35ug/lane). MEN1(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-MEN1 Antibody (T594) in Ramos cell line lysates (35ug/lane). MEN1(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MEN1 Antibody (T594) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MEN1 Antibody (T594) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MEN1 Antibody (T594) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MEN1 Antibody (T594) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Vidal,A., J Eur Acad Dermatol Venereol 22 (7), 835-838 (2008)
Pieterman,C.R., Clin. Endocrinol. (Oxf) (2008)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes menin, a putative tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. In vitro studies have shown menin is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD, however, the function of this protein is not known. Two messages have been detected on northern blots but the larger message has not been characterized. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]
Uniprot Description
MEN1: Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression. May be involved in DNA repair. Component of MLL-containing complexes (named MLL, ASCOM, MLL2/MLL3 or MLL3/MLL4 complex): at least composed ASH2L, RBBP5, DPY30, WDR5, one or several histone methyltransferases (MLL, MLL2, MLL3 and/or MLL4), and the facultative components MEN1, HCFC1, HCFC2, NCOA6, KDM6A, PAXIP1/PTIP and C16orf53/PA1. Interacts with POLR2A phosphorylated at 'Ser-5', but not with the unphosphorylated, nor 'Ser-2' phosphorylated POLR2A forms. Interacts with FANCD2 and DBF4. Interacts with JUND. Interacts with SMAD3, but not with SMAD2, nor SMAD4. Directly interacts with NFKB1, NFKB2 and RELA. Ubiquitous. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: nucleoplasm; histone methyltransferase complex; nuclear matrix; protein complex; cytoplasm; nucleus; chromatin; cytosol; cleavage furrow
Molecular Function: Y-form DNA binding; protein binding, bridging; protein binding; four-way junction DNA binding; sequence-specific DNA binding; double-stranded DNA binding; protein N-terminus binding; chromatin binding; histone-lysine N-methyltransferase activity
Biological Process: negative regulation of JNK cascade; maternal process involved in pregnancy; positive regulation of protein binding; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; palate development; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of protein amino acid phosphorylation; transforming growth factor beta receptor signaling pathway; response to gamma radiation; hemopoiesis; negative regulation of osteoblast differentiation; cell cycle arrest; response to UV; leukocyte homeostasis; transcription initiation from RNA polymerase II promoter; positive regulation of histone methylation; transcription, DNA-dependent; negative regulation of transcription factor activity; MAPKKK cascade; negative regulation of cyclin-dependent protein kinase activity; positive regulation of transforming growth factor beta receptor signaling pathway; negative regulation of organ growth; DNA repair; regulation of activin receptor signaling pathway; embryonic skeletal morphogenesis; osteoblast development; chromatin remodeling; negative regulation of telomerase activity; positive regulation of osteoblast differentiation; positive regulation of cell division; gene expression; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus; osteoblast fate commitment
Disease: Multiple Endocrine Neoplasia, Type I
Research Articles on MEN1
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Product Notes
The MEN1 men1 (Catalog #AAA9203393) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 584-615. The MEN1 Antibody (T594) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's MEN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the MEN1 men1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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