Western Blot (WB)
((0.1ug/ml) staining of human testis lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.)
Goat MELK Polyclonal Antibody | anti-MELK antibody
Goat anti-MELK Antibody
Western Blot: Experiments gave bands at approx 75kDa and 150kDa in human testis lysates and in cell lines HeLa and Jurkat after 0.1ug/ml antibody staining. The150kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6kDa according to NP_055606.1. Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of human testis lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of human testis lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
MELK: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis. Monomer. Interacts with ZNF622 and PPP1R8. Up-regulated in many cancers cells. Up-regulated upon treatment with radiation or 5-fluorouracil (5-FU) in colorectal cancer cells, suggesting that it might be associated with increased resistance of colorectal cells against radiation and 5- FU. Down-regulated upon siomycin A, a thiazole antibiotic, treatment, leading to inhibit tumor growth in vivo. Expressed in placenta, kidney, thymus, testis, ovary and intestine. Activated by autophosphorylation of the T-loop at Thr-167 and Ser-171: in contrast to other members of the SNF1 subfamily, phosphorylation at Thr-167 is not mediated by STK11/LKB1 but via autophosphorylation instead. Inhibited by calcium-binding. Kinase activity is also regulated by reducing agents: dithiothreitol (DTT) or reduced glutathione are required for kinase activity in vitro; such dependence is however not due to the presence of disulfide bonds. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Protein type: Protein kinase, CAMK; EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; RNA splicing; CAMK group; CAMKL family; MELK subfamily
Chromosomal Location of Human Ortholog: 9p13.2
Cellular Component: cell cortex; membrane; nucleus; plasma membrane
Molecular Function: ATP binding; calcium ion binding; lipid binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein serine/threonine kinase activity
Biological Process: apoptosis; cell proliferation; G2/M transition of mitotic cell cycle; hemopoiesis; induction of apoptosis by oxidative stress; peptidyl-tyrosine phosphorylation; positive regulation of apoptosis; protein amino acid autophosphorylation
Research Articles on MELK
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Product Notes
The MELK melk (Catalog #AAA421244) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MELK Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MELK can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Experiments gave bands at approx 75kDa and 150kDa in human testis lysates and in cell lines HeLa and Jurkat after 0.1ug/ml antibody staining. The150kDa band is explained by glycosylation of this member of the AMPK family. This protein has a calculated MW of 74.6kDa according to NP_055606.1. Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the MELK melk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KRLVEDILSS CKV. It is sometimes possible for the material contained within the vial of "MELK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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