Rabbit Mel18 Polyclonal Antibody | anti-Mel18 antibody

Mel18 (RNF110, DNA-binding protein Mel-18, RING finger protein 110, Zinc finger protein 144, polycomb group ring finger 2) (HRP)

Cat. Num. AAA6487671

• Gene Names: PCGF2; TPFS; MEL-18; RNF110; ZNF144
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: Mel18; Polyclonal Antibody; Mel18 (RNF110; DNA-binding protein Mel-18; RING finger protein 110; Zinc finger protein 144; polycomb group ring finger 2) (HRP); anti-Mel18 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RNF110/PCGF2. Species Crossreactivity: mouse, rat. Species sequence homology: bovine, horse, chimpanzee, macaque (100%).
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 344

• Applicable Applications for anti-Mel18 antibody: Western Blot (WB)
• Application Notes: WB: 1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Linear peptide corresponding to human RNF110/PCGF2 (KLH).
• Conjugate: HRP
• Positive Control: Human lung tissue lysate
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot Analysis: Representative lot data. Human Lung lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS621307 (1:1,000). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Mel18 (~40kD).)

Product Categories/Family for anti-Mel18 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

1. Suzuki, M., et al. (2002). Development. 129, 4171-4183.

NCBI and Uniprot Product Information

• NCBI GI #: 6005964
• NCBI GeneID: 7703
• NCBI Accession #: NP_009075
• NCBI GenBank Nucleotide #: NP_009075.1
• UniProt Accession #: P35227
• Molecular Weight: 40
• NCBI Official Full Name: polycomb group RING finger protein 2
• NCBI Official Synonym Full Names: polycomb group ring finger 2
• NCBI Official Symbol: PCGF2
• NCBI Official Synonym Symbols: TPFS; MEL-18; RNF110; ZNF144
• NCBI Protein Information: polycomb group RING finger protein 2
• UniProt Protein Name: Polycomb group RING finger protein 2
• UniProt Gene Name: PCGF2
• UniProt Synonym Gene Names: MEL18; RNF110; ZNF144
• UniProt Entry Name: PCGF2_HUMAN

NCBI Description

The protein encoded by this gene contains a RING finger motif and is similar to the polycomb group (PcG) gene products. PcG gene products form complexes via protein-protein interaction and maintain the transcription repression of genes involved in embryogenesis, cell cycles, and tumorigenesis. This protein was shown to act as a negative regulator of transcription and has tumor suppressor activity. The expression of this gene was detected in various tumor cells, but is limited in neural organs in normal tissues. Knockout studies in mice suggested that this protein may negatively regulate the expression of different cytokines, chemokines, and chemokine receptors, and thus plays an important role in lymphocyte differentiation and migration, as well as in immune responses. [provided by RefSeq, Jul 2008]