Rabbit MEF2A Polyclonal Antibody | anti-MEF2A antibody

MEF2A, Phosphorylated (T312) (Myocyte-specific Enhancer Factor 2A, Mef2, ADCAD1, RSRFC4, RSRFC9) (HRP)

Cat. Num. AAA6426478

• Gene Names: MEF2A; mef2; ADCAD1; RSRFC4; RSRFC9
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: MEF2A; Polyclonal Antibody; Phosphorylated (T312) (Myocyte-specific Enhancer Factor 2A; Mef2; ADCAD1; RSRFC4; RSRFC9) (HRP); anti-MEF2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes MEF2A, phosphorylated T312. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-MEF2A antibody: Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: IF: 1:100-1:200; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding T312 of human MEF2A.
• Conjugate: HRP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-MEF2A-T312 antibody.)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using Phospho-MEF2A-T312 antibody.)

Product Categories/Family for anti-MEF2A antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 195972801
• NCBI GeneID: 4205
• NCBI Accession #: NP_001124398.1
• NCBI GenBank Nucleotide #: NM_001130926.1
• UniProt Accession #: Q02078; O43814; Q14223; Q14224; Q59GX4; Q7Z6C9; Q96D14; B4DFQ7; F6XG23
• Molecular Weight: 54,811 Da
• NCBI Official Full Name: myocyte-specific enhancer factor 2A isoform 2
• NCBI Official Synonym Full Names: myocyte enhancer factor 2A
• NCBI Official Symbol: MEF2A
• NCBI Official Synonym Symbols: mef2; ADCAD1; RSRFC4; RSRFC9
• NCBI Protein Information: myocyte-specific enhancer factor 2A; serum response factor-like protein 1; MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A)
• UniProt Protein Name: Myocyte-specific enhancer factor 2A
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2A
• UniProt Synonym Gene Names: MEF2
• UniProt Entry Name: MEF2A_HUMAN

NCBI Description

The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]

Uniprot Description

MEF2A: a myocyte-specific enhancing transcription factor which binds specifically to the MEF2 element present in the regulatory regions of many, if not all, muscle-specific genes. A member of the MADS gene family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain. Five alternatively spliced isoforms have been reported.

Protein type: DNA-binding; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; protein binding; transcription activator binding; protein heterodimerization activity; sequence-specific DNA binding; histone deacetylase binding; chromatin binding; SMAD binding; transcription factor activity; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; muscle development; ventricular cardiac myofibril development; nerve growth factor receptor signaling pathway; apoptosis; heart development; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; mitochondrial genome maintenance; toll-like receptor 4 signaling pathway; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; dendrite morphogenesis; MAPKKK cascade; toll-like receptor 2 signaling pathway; mitochondrion distribution; MyD88-dependent toll-like receptor signaling pathway; muscle cell differentiation; toll-like receptor signaling pathway; positive regulation of muscle cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway

Disease: Coronary Artery Disease, Autosomal Dominant, 1