Rabbit MEF2A Polyclonal Antibody | anti-MEF2A antibody

Phospho-MEF2A (Thr312) Antibody

Cat. Num. AAA7133463

• Gene Names: MEF2A; mef2; ADCAD1; RSRFC4; RSRFC9
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
• Synonyms: MEF2A; Polyclonal Antibody; Phospho-MEF2A (Thr312) Antibody; MEF2; ADCAD1; RSRFC4; RSRFC9; anti-MEF2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
• Form/Format: Liquid; Supplied at 1.0mg/mL In Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol.

• Applicable Applications for anti-MEF2A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100; IF: 1:100-1:200
• Immunogen: Peptide sequence around phosphorylation site of threonine 312 (L-A-T(p)-P-V) derived from Human MEF2A.
• Immunogen Species: Human
• Conjugate: Non-conjugated
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEF2A(Phospho-Thr312) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using MEF2A(Phospho-Thr312) Antibody.)

Western Blot (WB)

(Western blot analysis of extracts from C2C12 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using MEF2A (Phospho-Thr312) Antibody.)

Product Categories/Family for anti-MEF2A antibody

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 195972801
• NCBI GeneID: 4205
• NCBI Accession #: NP_001124398.1
• NCBI GenBank Nucleotide #: NM_001130926.1
• UniProt Accession #: Q02078; O43814; Q14223; Q14224; Q59GX4; Q7Z6C9; Q96D14; B4DFQ7; F6XG23
• Molecular Weight: 54,811 Da
• NCBI Official Full Name: myocyte-specific enhancer factor 2A isoform 2
• NCBI Official Synonym Full Names: myocyte enhancer factor 2A
• NCBI Official Symbol: MEF2A
• NCBI Official Synonym Symbols: mef2; ADCAD1; RSRFC4; RSRFC9
• NCBI Protein Information: myocyte-specific enhancer factor 2A; serum response factor-like protein 1; MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A)
• UniProt Protein Name: Myocyte-specific enhancer factor 2A
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2A
• UniProt Synonym Gene Names: MEF2
• UniProt Entry Name: MEF2A_HUMAN

NCBI Description

The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]

Uniprot Description

MEF2A: a myocyte-specific enhancing transcription factor which binds specifically to the MEF2 element present in the regulatory regions of many, if not all, muscle-specific genes. A member of the MADS gene family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain. Five alternatively spliced isoforms have been reported.

Protein type: DNA-binding; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q26

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; protein binding; transcription activator binding; protein heterodimerization activity; sequence-specific DNA binding; histone deacetylase binding; chromatin binding; SMAD binding; transcription factor activity; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; muscle development; ventricular cardiac myofibril development; nerve growth factor receptor signaling pathway; apoptosis; heart development; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; mitochondrial genome maintenance; toll-like receptor 4 signaling pathway; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; dendrite morphogenesis; MAPKKK cascade; toll-like receptor 2 signaling pathway; mitochondrion distribution; MyD88-dependent toll-like receptor signaling pathway; muscle cell differentiation; toll-like receptor signaling pathway; positive regulation of muscle cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway

Disease: Coronary Artery Disease, Autosomal Dominant, 1