Rabbit anti-Human MED22 Polyclonal Antibody | anti-MED22 antibody

MED22, NT (MED22, SURF5, Mediator of RNA polymerase II transcription subunit 22, Mediator complex subunit 22, Surfeit locus protein 5) (AP)

Cat. Num. AAA6319990

• Gene Names: MED22; MED24; SURF5; surf-5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MED22; Polyclonal Antibody; NT (MED22; SURF5; Mediator of RNA polymerase II transcription subunit 22; Mediator complex subunit 22; Surfeit locus protein 5) (AP); Surfeit locus protein 5; anti-MED22 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-MED22 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MED22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-36 amino acids from the N-terminal region of human MED22.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MED22 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MED22 antibody detected the MED22 protein (arrow).)

Related Product Information for anti-MED22 antibody

This gene is located in the surfeit gene cluster, a group of very tightly linked housekeeping genes that do not share sequence similarity. The gene is oriented in a head-to-head fashion with RPL7A (SURF3) and the two genes share a bidirectional promoter. The encoded proteins are localized to the cytoplasm. Two alternative transcript variants encoding different isoforms have been identified for this gene.

Product Categories/Family for anti-MED22 antibody

Antibodies; Enzymes; Polymerase

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007). Sato, S., et al. Mol. Cell 14(5):685-691(2004). Hillman, R.T., et al. Genome Biol. 5 (2), R8 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 19557695
• NCBI GeneID: 6837
• NCBI Accession #: NP_598395.1; NP_852468.1
• NCBI GenBank Nucleotide #: NM_133640.4
• UniProt Accession #: Q15528; O76072; Q5T8U0; B3KW83; B3KWX4
• Molecular Weight: 16,480 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 22 isoform b
• NCBI Official Synonym Full Names: mediator complex subunit 22
• NCBI Official Symbol: MED22
• NCBI Official Synonym Symbols: MED24; SURF5; surf-5
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 22; surfeit locus protein 5
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 22
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED22
• UniProt Synonym Gene Names: SURF5; Surf-5
• UniProt Entry Name: MED22_HUMAN

NCBI Description

This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013]

Uniprot Description

SURF5: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 22 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9q34.2

Cellular Component: cytoplasm; Srb-mediator complex

Molecular Function: protein binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter