Rabbit anti-Human MED14 Polyclonal Antibody | anti-MED14 antibody

MED14 Antibody

Cat. Num. AAA9403504

• Gene Names: MED14; CSRP; RGR1; CRSP2; EXLM1; CXorf4; CRSP150; DRIP150; TRAP170
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: MED14; Polyclonal Antibody; MED14 Antibody; ARC150; Activator-recruited cofactor 150 kDa component; CRSP2; CSP2; Cofactor required for Sp1 transcriptional activation subunit 2; anti-MED14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total MED14 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1454

• Applicable Applications for anti-MED14 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:500
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human MED14.
• Target Name: MED14
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MED14 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of COS-7 cells, using MED14 antibody.)

Related Product Information for anti-MED14 antibody

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.Yoshikawa H., Genomics 49:237-246(1998).Ryu S., Nature 397:446-450(1999).Rachez C., Nature 398:824-828(1999).

Product Categories/Family for anti-MED14 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 28558973
• NCBI GeneID: 9282
• NCBI Accession #: NP_004220.2
• NCBI GenBank Nucleotide #: NM_004229.3
• UniProt Accession #: O60244; Q4KMR7; Q9UNB3
• Molecular Weight: 160,607 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 14
• NCBI Official Synonym Full Names: mediator complex subunit 14
• NCBI Official Symbol: MED14
• NCBI Official Synonym Symbols: CSRP; RGR1; CRSP2; EXLM1; CXorf4; CRSP150; DRIP150; TRAP170
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 14
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 14
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED14
• UniProt Synonym Gene Names: ARC150; CRSP2; CXorf4; DRIP150; EXLM1; RGR1; TRAP170; ARC150; CRSP complex subunit 2; hRGR1; Trap170; DRIP150
• UniProt Entry Name: MED14_HUMAN

NCBI Description

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq, Jul 2008]

Uniprot Description

Trap170: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 14 family.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: nucleoplasm; membrane; Srb-mediator complex; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity; vitamin D receptor binding; transcription coactivator activity; transcription cofactor activity; thyroid hormone receptor binding; receptor activity

Biological Process: steroid hormone receptor signaling pathway; regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; stem cell maintenance; gene expression; positive regulation of transcription from RNA polymerase II promoter