Rabbit anti-Human, Mouse MED13L Polyclonal Antibody | anti-MED13L antibody

MED13L Antibody

Cat. Num. AAA9630078

• Gene Names: MED13L; THRAP2; TRAP240L; PROSIT240
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MED13L; Polyclonal Antibody; MED13L Antibody; MD13L_HUMAN; Mediator complex subunit 13 like; Mediator complex subunit 13-like; Mediator of RNA polymerase II transcription subunit 13-like; THRAP2; Thyroid hormone receptor-associated protein 2; Thyroid hormone receptor-associated protein complex 240 kDa component-like; TRAP240L; anti-MED13L antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: MED13L Antibody detects endogenous levels of MED13L.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-MED13L antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human MED13L.
• Conjugation: Unconjugated
• Similarity: Belongs to the Mediator complex subunit 13 family.
• Subunit Structure: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
• Tissue Specificity: Highly expressed in brain (cerebellum), heart (aorta), skeletal muscle, kidney, placenta and peripheral blood leukocytes. Highly expressed in fetal brain.
• Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-MED13L antibody

Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 44771211
• NCBI GeneID: 23389
• NCBI Accession #: NP_056150.1
• NCBI GenBank Nucleotide #: NM_015335.4
• UniProt Accession #: Q71F56; Q68DN4; Q9H8C0; Q9NSY9; Q9UFD8; Q9UPX5; A1L469
• Molecular Weight: 242,602 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 13-like
• NCBI Official Synonym Full Names: mediator complex subunit 13-like
• NCBI Official Symbol: MED13L
• NCBI Official Synonym Symbols: THRAP2; TRAP240L; PROSIT240
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 13-like; thyroid hormone receptor-associated protein 2; thyroid hormone receptor-associated protein complex 240 kDa component-like
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 13-like
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED13L
• UniProt Synonym Gene Names: KIAA1025; PROSIT240; THRAP2; TRAP240L
• UniProt Entry Name: MD13L_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the Mediator complex, a large complex of proteins that functions as a transcriptional coactivator for most RNA polymerase II-transcribed genes. The encoded protein is involved in early development of the heart and brain. Defects in this gene are a cause of transposition of the great arteries, dextro-looped (DTGA).[provided by RefSeq, Jul 2010]

Uniprot Description

THRAP2: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Defects in MED13L are a cause of transposition of the great arteries dextro-looped type 1 (DTGA1). A congenital heart defect consisting of complete inversion of the great vessels, so that the aorta incorrectly arises from the right ventricle and the pulmonary artery incorrectly arises from the left ventricle. This creates completely separate pulmonary and systemic circulatory systems, an arrangement that is incompatible with life. Patients often have atrial and/or ventricular septal defects or other types of shunting that allow some mixing between the circulations in order to support life minimally, but surgical intervention is always required. A chromosomal aberration involving MED13L is found in a patient with transposition of the great arteries, dextro- looped and mental retardation. Translocation t(12;17)(q24.1;q21). Belongs to the Mediator complex subunit 13 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 12q24.21

Cellular Component: Srb-mediator complex

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter

Disease: Transposition Of The Great Arteries, Dextro-looped 1