Western Blot (WB)
(MeCP2 pAb tested by Western blot. HeLa acid extract (10 ug per lane) was probed with MeCP2 pAb (1:25,000 dilution).)
Rabbit anti-Human, Mouse MeCP2 Polyclonal Antibody | anti-MeCP2 antibody
MeCP2 antibody (pAb)
Reactivity
Human, Mouse
Applications
Western Blot
Synonyms
MeCP2; Polyclonal Antibody; MeCP2 antibody (pAb); Methyl-CpC binding protein 2; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13; anti-MeCP2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Form/Format
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-MeCP2 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:10,000-1:50,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
WB: 1:10,000-1:50,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This MeCP2 antibody was raised against recombinant protein corresponding to the amino-terminal 320 amino acids of mouse MeCP2.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(MeCP2 pAb tested by Western blot. HeLa acid extract (10 ug per lane) was probed with MeCP2 pAb (1:25,000 dilution).)
Western Blot (WB)
(MeCP2 pAb tested by Western blot. HeLa acid extract (10 ug per lane) was probed with MeCP2 pAb (1:25,000 dilution).)
Related Product Information for anti-MeCP2 antibody
Short Description: Our MeCP2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human and Mouse samples.
Background: Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MeCP2 (methyl-CpG binding protein 2) can repress transcription by recruiting the Sin3 complex including histone deacetylases (HDACs). MeCP2 is distributed throughout the nucleus in human cells.
Background: Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and control of gene expression. It is generally associated with the repressive chromatin state. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins include MeCP2, MBD1, MBD2, MBD3 and MBD4. MeCP2 (methyl-CpG binding protein 2) can repress transcription by recruiting the Sin3 complex including histone deacetylases (HDACs). MeCP2 is distributed throughout the nucleus in human cells.
Product Categories/Family for anti-MeCP2 antibody
NCBI and Uniprot Product Information
Molecular Weight
75kDa
Protein Family
Similar Products
Product Notes
The MeCP2 (Catalog #AAA388394) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MeCP2 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MeCP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:10,000-1:50,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the MeCP2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MeCP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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