Rabbit anti-Human MDM2 Polyclonal Antibody | anti-MDM2 antibody

MDM2 (Murine Double Minute-2) (MaxLight 550)

Cat. Num. AAA6487454

• Gene Names: MDM2; HDMX; hdm2; ACTFS
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MDM2; Polyclonal Antibody; MDM2 (Murine Double Minute-2) (MaxLight 550); anti-MDM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MDM2 at 76kD.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-MDM2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.; WB: 2-4ug/ml detects MDM2 in RIPA lysates from HeLa cells. Hela cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M2800-03B
• Immunogen: Synthetic peptide corresponding to amino acids 164-185 of human MDM2, CAISETEENSDELSGERQRKRHK (KLH) with an N-terminal cysteine added for conjugation purposes. The immunizing peptide is identical in both p90MDM2 and p76MDM2.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(WB: Hela cell lysate was probed with M2800-03B anti-MDM2 (2ug/ml). )

Product Categories/Family for anti-MDM2 antibody

Antibodies; Ubiquitin

References

1. Leveillard, T. and B. Wasylyk, J. Biol. Chem. 272: 30651-30661, 1997.

NCBI and Uniprot Product Information

• NCBI GI #: 89993689
• NCBI GeneID: 4193
• NCBI Accession #: NP_002383.2
• NCBI GenBank Nucleotide #: NM_002392
• UniProt Accession #: Q00987; Q13226; Q13297; Q13298; Q13299; Q13300; Q13301; Q53XW0; Q71TW9; Q8WYJ1; A6NL51; A8K2S6
• Molecular Weight: 55,233 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase Mdm2 isoform a
• NCBI Official Synonym Full Names: MDM2 oncogene, E3 ubiquitin protein ligase
• NCBI Official Symbol: MDM2
• NCBI Official Synonym Symbols: HDMX; hdm2; ACTFS
• NCBI Protein Information: E3 ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; Mdm2, p53 E3 ubiquitin protein ligase homolog; double minute 2, human homolog of; p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein
• UniProt Protein Name: E3 ubiquitin-protein ligase Mdm2
• Protein Family: Mdm2-binding protein
• UniProt Gene Name: MDM2
• UniProt Synonym Gene Names: Hdm2
• UniProt Entry Name: MDM2_HUMAN

NCBI Description

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013]

Uniprot Description

MDM2: a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 via Akt-mediated MDM2 phosphorylation. Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53. Facilitates the nuclear export of p53 and targets it for proteasome-mediated proteolysis. Eight alternatively spliced isoforms have been reported.

Protein type: Ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase; Oncoprotein; Inhibitor; Ubiquitin conjugating system; Nucleolus

Chromosomal Location of Human Ortholog: 12q14.3-q15

Cellular Component: nucleoplasm; nuclear body; protein complex; cytoplasm; nucleolus; plasma membrane; synapse; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; peroxisome proliferator activated receptor binding; enzyme binding; zinc ion binding; p53 binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; response to morphine; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to carbohydrate stimulus; response to antibiotic; positive regulation of protein export from nucleus; synaptic transmission; negative regulation of DNA damage response, signal transduction by p53 class mediator; response to magnesium ion; establishment of protein localization; peptidyl-lysine modification; positive regulation of cell proliferation; protein complex assembly; response to iron ion; epidermal growth factor receptor signaling pathway; response to drug; traversing start control point of mitotic cell cycle; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; protein destabilization; response to ether; response to cocaine; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; innate immune response; regulation of protein catabolic process; negative regulation of transcription, DNA-dependent

Disease: Accelerated Tumor Formation, Susceptibility To