Rabbit anti-Human, Mouse MDFIC Polyclonal Antibody | anti-MDFIC antibody

MDFIC Antibody

Cat. Num. AAA9631317

• Gene Names: MDFIC; HIC
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MDFIC; Polyclonal Antibody; MDFIC Antibody; HIC; Human I mfa domain containing protein; Human I-mfa domain-Containing protein; I mfa domain containing protein; MyoD family inhibitor domain containing protein; MyoD family inhibitor domain-containing protein; p32; p40; anti-MDFIC antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: MDFIC Antibody detects endogenous levels of MDFIC.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-MDFIC antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human MDFIC.
• Conjugation: Unconjugated
• Similarity: The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation (By similarity). The cysteine-rich C-terminus is involved in its granular distribution in the cytoplasm. Belongs to the MDFI family.
• Subunit Structure: Interacts with HAND1; leading to sequester HAND1 into the nucleolus and prevent its activity. Interacts with ZIC2 (By similarity). The C-terminus interacts with AXIN1, the histidine-rich region of CCNT1/cyclin-T and weakly with LEF1. Interacts with CCNT2.(Microbial infection) Interacts (via C-terminus) with HIV-1 Tat and Rev.
• Tissue Specificity: Expressed in lymphoid organs (spleen, thymus, peripheral blood leukocytes) as well as prostate, uterus and small intestine.
• Function: Acts as a transcriptional activator or repressor. Inhibits the transcriptional activation of Zic family proteins ZIC1, ZIC2 and ZIC3. Retains nuclear Zic proteins ZIC1, ZIC2 and ZIC3 in the cytoplasm. Modulates the expression from both cellular and viral promoters. Down-regulates Tat-dependent transcription of the human immunodeficiency virus type 1 (HIV-1) LTR by interacting with HIV-1 Tat and Rev and impairing their nuclear import, probably by rendering the NLS domains inaccessible to importin-beta. Also stimulates activation of human T-cell leukemia virus type I (HTLV-I) LTR. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-MDFIC antibody

Nucleus; Primary; Cytoplasm

NCBI and Uniprot Product Information

• NCBI GI #: 40018612
• NCBI GeneID: 29969
• NCBI Accession #: NP_951038.1
• NCBI GenBank Nucleotide #: NM_199072.4
• UniProt Accession #: Q9P1T7; Q9P1T6
• Molecular Weight: 25,788 Da
• NCBI Official Full Name: myoD family inhibitor domain-containing protein isoform p40
• NCBI Official Synonym Full Names: MyoD family inhibitor domain containing
• NCBI Official Symbol: MDFIC
• NCBI Official Synonym Symbols: HIC
• NCBI Protein Information: myoD family inhibitor domain-containing protein; I-mfa domain-containing protein
• UniProt Protein Name: MyoD family inhibitor domain-containing protein
• Protein Family: MyoD family inhibitor domain-containing protein
• UniProt Gene Name: MDFIC
• UniProt Synonym Gene Names: hIC
• UniProt Entry Name: MDFIC_HUMAN

NCBI Description

This gene product is a member of a family of proteins characterized by a specific cysteine-rich C-terminal domain, which is involved in transcriptional regulation of viral genome expression. Alternative translation initiation from an upstream non-AUG (GUG), and an in-frame, downstream AUG codon, results in the production of two isoforms, p40 and p32, respectively, which have different subcellular localization; p32 is mainly found in the cytoplasm, whereas p40 is targeted to the nucleolus. Both isoforms have transcriptional regulatory activity that is attributable to the cysteine-rich C-terminal domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

MDFIC: Acts as a transcriptional activator or repressor. Inhibits the transcriptional activation of Zic family proteins ZIC1, ZIC2 and ZIC3. Retains nuclear Zic proteins ZIC1, ZIC2 and ZIC3 in the cytoplasm. Modulates the expression from both cellular and viral promoters. Down-regulates Tat-dependent transcription of the human immunodeficiency virus type 1 (HIV-1) LTR by interacting with HIV-1 Tat and Rev and impairing their nuclear import, probably by rendering the NLS domains inaccessible to importin- beta. Also stimulates activation of human T-cell leukemia virus type I (HTLV-I) LTR. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways. Belongs to the MDFI family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 7q31.1-q31.2

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleolus; nucleus

Molecular Function: cyclin binding; protein binding; Tat protein binding; transcription factor binding

Biological Process: transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; positive regulation of transcription, DNA-dependent; regulation of Wnt receptor signaling pathway; negative regulation of protein import into nucleus; negative regulation of transcription, DNA-dependent; activation of JNK activity