Rabbit MDFI Polyclonal Antibody | anti-MDFI antibody

MDFI (P134) Polyclonal Antibody

Cat. Num. AAA3004420

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MDFI; Polyclonal Antibody; MDFI (P134) Polyclonal Antibody; MyoD family inhibitor; Myogenic repressor I-mf; anti-MDFI antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MDFI (P134) polyclonal antibody detects endogenous levels of MDFI protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 246

• Applicable Applications for anti-MDFI antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 100-146 of Human MDFI.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MDFI (P134) pAb at 1:500 dilutionLane1:SGC7901 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:C6 whole cell lysate (40ug) Lane6:3T3-L1 whole cell lysate (40ug))

Related Product Information for anti-MDFI antibody

MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. MDFI an also interfere with the DNA-binding activity of Myod family members and plays an important role in trophoblast and chondrogenic differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 5031905
• NCBI Accession #: NP_005577.1
• NCBI GenBank Nucleotide #: NP_005577.1
• UniProt Accession #: Q99750
• Molecular Weight: ~ 25kDa
• NCBI Official Full Name: myoD family inhibitor isoform 1
• UniProt Protein Name: MyoD family inhibitor
• Protein Family: MyoD family inhibitor
• UniProt Gene Name: MDFI
• UniProt Entry Name: MDFI_HUMAN

Uniprot Description

MDFI: Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways. Belongs to the MDFI family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; transcription factor binding

Biological Process: embryonic development; cytoplasmic sequestering of transcription factor; negative regulation of Wnt receptor signaling pathway; dorsal/ventral axis specification; negative regulation of transcription from RNA polymerase II promoter; negative regulation of DNA binding; activation of JNK activity; embryonic skeletal morphogenesis