Goat anti-Human MCP4 Polyclonal Antibody | anti-CCL13 antibody

MCP4 (Macrophage/Monocyte Chemotactic Protein 4)

Cat. Num. AAA618818

• Gene Names: CCL13; NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13; MGC17134
• Reactivity: Human
• Applications: ELISA, Western Blot, Neutralization Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: MCP4; Polyclonal Antibody; MCP4 (Macrophage/Monocyte Chemotactic Protein 4); Anti -MCP4 (Macrophage/Monocyte Chemotactic Protein 4); anti-CCL13 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MCP-4.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder.

• Applicable Applications for anti-CCL13 antibody: ELISA (EL/EIA), Western Blot (WB), Neutralization
• Application Notes: Suitable for use in ELISA, Western Blot and Neutralization.; Dilution: ELISA: To detect hMCP-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant hMCP-4.; Western Blot: To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.; Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-4 (100ng/ml), a concentration of 8-12ug/ml of this antibody is required.
• Immunogen: Highly pure (> 98%) recombinant human MCP-4
• Country of Origin: USA
• Reconstitution: Reconstitute to 0.2mg/ml with sterile dH2O or sterile 40-50% glycerol for long term storage.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-CCL13 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 4097421
• NCBI GeneID: 6357
• UniProt Accession #: Q99616; O95689; Q6ICQ6; O95690
• Molecular Weight: 10,986 Da
• NCBI Official Full Name: monocyte chemotactic protein-4
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 13
• NCBI Official Symbol: CCL13
• NCBI Official Synonym Symbols: NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13; MGC17134
• NCBI Protein Information: C-C motif chemokine 13; CK-beta-10; OTTHUMP00000163804; new CC chemokine 1; small-inducible cytokine A13; monocyte chemotactic protein 4; monocyte chemoattractant protein 4; small inducible cytokine subfamily A (Cys-Cys), member 13
• UniProt Protein Name: C-C motif chemokine 13
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL13
• UniProt Synonym Gene Names: MCP4; NCC1; SCYA13
• UniProt Entry Name: CCL13_HUMAN

NCBI Description

This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. This chemokine plays a role in accumulation of leukocytes during inflammation. It may also be involved in the recruitment of monocytes into the arterial wall during artherosclerosis. [provided by RefSeq]

Uniprot Description

CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine; Secreted

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: extracellular space

Molecular Function: chemokine activity; receptor binding

Biological Process: cellular calcium ion homeostasis; regulation of cell shape; cell-cell signaling; eosinophil chemotaxis; cytoskeleton organization and biogenesis; immune response; signal transduction; chemotaxis; inflammatory response