Rabbit anti-Human, Mouse MCM4 Polyclonal Antibody | anti-MCM4 antibody

MCM4 (Phospho-Ser54) Antibody

Cat. Num. AAA9502741

• Gene Names: MCM4; NKCD; CDC21; CDC54; IMD54; NKGCD; hCdc21; P1-CDC21
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: MCM4; Polyclonal Antibody; MCM4 (Phospho-Ser54) Antibody; CDC21 homolog; DNA replication licensing factor MCM4; P1-CDC21; anti-MCM4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: MCM4 (Phospho-Ser54) Antibody detects endogenous levels of MCM4 only when phosphorylated at Ser54.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 863

• Applicable Applications for anti-MCM4 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:20000
• Target Modification: Phospho
• Modification Sites: H:S54; M:S53
• Immunogen: The antiserum was produced against synthesized peptide derived from human MCM4 around the phosphorylation site of Ser54.
• Immunogen Range: 20-69
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysates from COS7 cells treated with nocodazole 1ug/ml 16h, using MCM4 (Phospho-Ser54) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 33469919
• NCBI GeneID: 4173
• NCBI Accession #: NP_005905.2
• NCBI GenBank Nucleotide #: NM_005914.3
• UniProt Accession #: P33991; Q8NEH1; Q99658
• Molecular Weight: 96 kDa
• NCBI Official Full Name: DNA replication licensing factor MCM4
• NCBI Official Synonym Full Names: minichromosome maintenance complex component 4
• NCBI Official Symbol: MCM4
• NCBI Official Synonym Symbols: NKCD; CDC21; CDC54; IMD54; NKGCD; hCdc21; P1-CDC21
• NCBI Protein Information: DNA replication licensing factor MCM4
• UniProt Protein Name: DNA replication licensing factor MCM4
• Protein Family: DNA replication licensing factor
• UniProt Gene Name: MCM4
• UniProt Synonym Gene Names: CDC21

NCBI Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.