Rabbit anti-Human MCL1 Polyclonal Antibody | anti-MCL1 antibody

MCL1, ID (MCL1, BCL2L3, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT) (APC)

Cat. Num. AAA6319651

• Gene Names: MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MCL1; Polyclonal Antibody; ID (MCL1; BCL2L3; Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT) (APC); mcl1/EAT; anti-MCL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MCL1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 271

• Applicable Applications for anti-MCL1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 217-245 in the central region of human MCL1, UniProt Accession #Q07820; NP_877495.1; NP_068779.1; Q9Z1P3. Species sequence homology: rat
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Y79 cell line lysates (35ug/lane) usingMBS6002192. This demonstrates that MBS6002192 detected the MCL1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue using MBS6002192 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6002192 for immunohistochemistry.)

Related Product Information for anti-MCL1 antibody

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing.

Product Categories/Family for anti-MCL1 antibody

Antibodies; Cancer Markers; Leukemia

References

1. Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. Kim, J.H., et al. FEBS Lett. 583(17):2758-2764(2009). 4. Maurer, U., et al. Mol. Cell 21(6):749-760(2006). 5. Domina, A.M., et al. Oncogene 23(31):5301-5315(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 33519458
• NCBI GeneID: 4170
• NCBI Accession #: NP_877495.1; NP_068779.1
• NCBI GenBank Nucleotide #: NP_877495.1
• UniProt Accession #: Q07820; Q9Z1P3
• NCBI Official Full Name: induced myeloid leukemia cell differentiation protein Mcl-1 isoform 2
• NCBI Official Synonym Full Names: MCL1 apoptosis regulator, BCL2 family member
• NCBI Official Symbol: MCL1
• NCBI Official Synonym Symbols: TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• NCBI Protein Information: induced myeloid leukemia cell differentiation protein Mcl-1
• UniProt Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1
• Protein Family: Minichromosome loss protein
• UniProt Gene Name: MCL1
• UniProt Synonym Gene Names: BCL2L3; Bcl2-L-3
• UniProt Entry Name: MCL1_HUMAN

NCBI Description

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

Uniprot Description

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Membrane protein, integral; Mitochondrial; Channel, misc.; Inhibitor; Apoptosis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding

Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; multicellular organismal development; cellular homeostasis; cell fate determination