Rabbit MCCC2 Polyclonal Antibody | anti-MCCC2 antibody

MCCC2 Antibody

Cat. Num. AAA9611407

• Gene Names: MCCC2; MCCB
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MCCC2; Polyclonal Antibody; MCCC2 Antibody; 3 methylcrotonyl CoA carboxylase 2; 3 methylcrotonyl CoA carboxylase non biotin containing subunit; 3 methylcrotonyl CoA:carbon dioxide ligase subunit beta; 3-methylcrotonyl-CoA carboxylase 2; 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta; Biotin carboxylase; MCCase subunit beta; MCCB; MCCB_HUMAN; MCCC 2; Mccc2; Methylcrotonoyl CoA carboxylase 2 (beta); Methylcrotonoyl CoA carboxylase beta chain mitochondrial; Methylcrotonoyl Coenzyme A carboxylase 2 (beta); Methylcrotonoyl-CoA carboxylase beta chain; mitochondrial; Non biotin containing subunit of 3 methylcrotonyl CoA carboxylase; anti-MCCC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MCCC2 Antibody detects endogenous levels of MCCC2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 563

• Applicable Applications for anti-MCCC2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; IFF/ICC: 1:100-1:500; The optimal dilution should be determined by the end user.; IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Immunogen: A synthesized peptide derived from human MCCC2.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Human liver tissue, using MCCC2 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from Hela cells, using MCCC2 Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)

(MBS9611407 staining Hela cells by IF/ICC.The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C.Samples were then incubated with primary Ab(MBS9611407 1:200) and mouse antibeta tubulin Ab (MBS9610328 1:200) for 1 hour at 37°C.An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)

Related Product Information for anti-MCCC2 antibody

Description: Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.

NCBI and Uniprot Product Information

• NCBI GI #: 11545863
• NCBI GeneID: 64087
• NCBI Accession #: NP_071415.1
• NCBI GenBank Nucleotide #: NP_071415.1
• UniProt Accession #: Q9HCC0
• Molecular Weight: Observed: 61 kDa; Predicted: 62 kDa
• NCBI Official Full Name: methylcrotonoyl-CoA carboxylase beta chain, mitochondrial isoform 1
• NCBI Official Synonym Full Names: methylcrotonoyl-CoA carboxylase 2
• NCBI Official Symbol: MCCC2
• NCBI Official Synonym Symbols: MCCB
• NCBI Protein Information: methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
• UniProt Protein Name: Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial
• Protein Family: Methylcrotonoyl-CoA carboxylase
• UniProt Gene Name: MCCC2
• UniProt Synonym Gene Names: MCCB; MCCase subunit beta
• UniProt Entry Name: MCCB_HUMAN

NCBI Description

This gene encodes the small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]