Rabbit Matrix Metalloproteinase 9 Polyclonal Antibody | anti-MMP-9 antibody

Matrix Metalloproteinase 9 (MMP-9, Collagenase 4, Gelatinase B) (FITC)

Cat. Num. AAA6487032

• Gene Names: MMP9; GELB; CLG4B; MMP-9; MANDP2
• Reactivity: Rat, Human, Mouse
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Matrix Metalloproteinase 9; Polyclonal Antibody; Matrix Metalloproteinase 9 (MMP-9; Collagenase 4; Gelatinase B) (FITC); anti-MMP-9 antibody

• Host: Rabbit
• Reactivity: Rat, Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Matrix Metalloproteinase 9 pro and active forms. Exhibits no crossreactivity with other MMP family members. Species Crossreactivity: mouse and human
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-MMP-9 antibody: Immunohistochemistry (IHC) Frozen, Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IHC-F: 1:100 in frozen acetone fixed tissues.; WB: 1:1000 detected MMP-9 on 10ug of L6 lysates at ~98kD.; IP: 1:500; Applications are based on unconjugated antibody.
• Immunogen: E. coli expressed active rat 92kD type IV collagenase (catalytic domain) (Accession# NP_004985).
• Conjugate: FITC
• Positive Control: HL60 cell lysate, U937 cell lysate lung
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Testing Data

(C2C12 lysate (Lane 1), L6 lysate (Lane 2), NIH/3T3 lysate (Lane 3), PC12 lysate (Lane 4), PC3 lysate (Lane 5), RAW264.7 lysate (Lane 6) were resolved by electrophoresis, transferred to PVDF and probed with M2425-31. Proteins were visulalized using a donkey anti-rabbit secondary antobody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MMP-9 at ~98kD.)

Product Categories/Family for anti-MMP-9 antibody

Antibodies; Matrix Metalloproteinases

References

1. Greenwald, R.A., Ann. NY Acad. Sci. 732: 273-179 (1994). 2. Van wart, H.E., et al., Proc. Natl. Acad. Sci. USA. 87: 5578-5582 (1990). 3. Goldberg, G.I., et al., J. Biol. Chem. 267: 4583-4591 (1992). 4. Christopolous, T.A., et al., Inter. J. Oncology 24: 1-5 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 74272287
• NCBI GeneID: 4318
• NCBI Accession #: NP_004985
• NCBI GenBank Nucleotide #: NM_004994.2
• UniProt Accession #: P14780; Q3LR70; Q8N725; Q9H4Z1; Q9UCJ9; Q9UCL1; Q9UDK2; B2R7V9
• Molecular Weight: Predicted: 76 kDa; Observed: 73kDa
• NCBI Official Full Name: matrix metalloproteinase-9 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• NCBI Official Symbol: MMP9
• NCBI Official Synonym Symbols: GELB; CLG4B; MMP-9; MANDP2
• NCBI Protein Information: matrix metalloproteinase-9; type V collagenase; macrophage gelatinase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• UniProt Protein Name: Matrix metalloproteinase-9
• UniProt Gene Name: MMP9
• UniProt Synonym Gene Names: CLG4B; MMP-9; GELB
• UniProt Entry Name: MMP9_HUMAN

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

Uniprot Description

MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as heterodimer with a 25 kDa protein. Macrophages and transformed cell lines produce only the monomeric form. Interacts with ECM1. Activated by 4-aminophenylmercuric acetate and phorbol ester. Up-regulated by ARHGEF4, SPATA13 and APC via the JNK signaling pathway in colorectal tumor cells. Produced by normal alveolar macrophages and granulocytes. Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1. Belongs to the peptidase M10A family.

Protein type: Protease; EC 3.4.24.35; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: collagen binding; identical protein binding; protein binding; zinc ion binding; metalloendopeptidase activity; endopeptidase activity

Biological Process: positive regulation of keratinocyte migration; axon guidance; ossification; extracellular matrix organization and biogenesis; macrophage differentiation; positive regulation of apoptosis; proteolysis; extracellular matrix disassembly; collagen catabolic process; ephrin receptor signaling pathway; skeletal development; leukocyte migration; embryo implantation

Disease: Metaphyseal Anadysplasia 2