Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Matrix Metalloproteinase 15 (MMP15) Polyclonal Antibody | anti-MMP15 antibody
Polyclonal Antibody to Matrix Metalloproteinase 15 (MMP15)
Gene Names
Mmp15; MT2-MMP; AI503551; MT-MMP 2
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Matrix Metalloproteinase 15 (MMP15); Polyclonal Antibody; Polyclonal Antibody to Matrix Metalloproteinase 15 (MMP15); anti-MMP15 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MMP15. It has been selected for its ability to recognize MMP15 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PGGKP ERPPKPGPPP QPRATERPDQ YGPNICDGNF DTVAVLRGEM FVFKGRWFWR VRHNRVLDNY PMPIGHFWRG LPGNISAAYE RQDGHFVFFK GNRYWLFREA NLEPGYPQPL SSYGTDIPYD RIDTAIWWEP TGHTFFFQAD RYWRFNEETQ HGDPGYPKPI SVWQGIPTSP KGAFLSNDAA
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PGGKP ERPPKPGPPP QPRATERPDQ YGPNICDGNF DTVAVLRGEM FVFKGRWFWR VRHNRVLDNY PMPIGHFWRG LPGNISAAYE RQDGHFVFFK GNRYWLFREA NLEPGYPQPL SSYGTDIPYD RIDTAIWWEP TGHTFFFQAD RYWRFNEETQ HGDPGYPKPI SVWQGIPTSP KGAFLSNDAA
Sequence Length
669
Applicable Applications for anti-MMP15 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant MMP15 (Pro336~Arg539) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2041072
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74,666 Da
NCBI Official Full Name
matrix metalloproteinase-15 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 15
NCBI Official Symbol
Mmp15
NCBI Official Synonym Symbols
MT2-MMP; AI503551; MT-MMP 2
NCBI Protein Information
matrix metalloproteinase-15
UniProt Protein Name
Matrix metalloproteinase-15
Protein Family
UniProt Gene Name
Mmp15
UniProt Synonym Gene Names
MMP-15; MT-MMP 2; MTMMP2; MT2-MMP; MT2MMP
NCBI Description
This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. A deficiency of the encoded protein in mice is compatible with normal development and postnatal growth. [provided by RefSeq, Feb 2016]
Uniprot Description
MMP15: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A. Belongs to the peptidase M10A family.
Protein type: EC 3.4.24.-; Membrane protein, integral; Protease
Chromosomal Location of Human Ortholog: 8 C5|8 47.12 cM
Cellular Component: extracellular matrix; integral component of membrane; integral component of plasma membrane; membrane
Molecular Function: hydrolase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding
Biological Process: proteolysis
Research Articles on MMP15
Similar Products
Product Notes
The MMP15 mmp15 (Catalog #AAA2015357) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Matrix Metalloproteinase 15 (MMP15) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Matrix Metalloproteinase 15 (MMP15) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MMP15 mmp15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PGGKP ERPPKPGPPP QPRATERPDQ YGPNICDGNF DTVAVLRGEM FVFKGRWFWR VRHNRVLDNY PMPIGHFWRG LPGNISAAYE RQDGHFVFFK GNRYWLFREA NLEPGYPQPL SSYGTDIPYD RIDTAIWWEP TGHTFFFQAD RYWRFNEETQ HGDPGYPKPI SVWQGIPTSP KGAFLSNDAA. It is sometimes possible for the material contained within the vial of "Matrix Metalloproteinase 15 (MMP15), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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