Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded prostate carcinoma tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of Anti-MT1-MMP, catalytic domain. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears to be restricted to the plasma membrane of malignant cells)
Rabbit anti-Human Matrix Metalloproteinase 14 Polyclonal Antibody | anti-MMP-14 antibody
Matrix Metalloproteinase 14, Catalytic Domain, active (MT1-MMP, MMP-14, Collagenase-3) Recombinant (MaxLight 750)
WB: 1ug/ml
IP: 10ug
Applications are based on unconjugated antibody.
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded prostate carcinoma tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of Anti-MT1-MMP, catalytic domain. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears to be restricted to the plasma membrane of malignant cells)
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Representative lot data. Paraffin-embedded prostate carcinoma tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of Anti-MT1-MMP, catalytic domain. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears to be restricted to the plasma membrane of malignant cells)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. HT-1080 cell lysate was probed with Anti-MT1-MMP, catalytic domain (1 ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. Arrow indicates MT1-MMP (~ 63 kD).)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. HT-1080 cell lysate was probed with Anti-MT1-MMP, catalytic domain (1 ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and detected using a chemiluminescence detection system. Arrow indicates MT1-MMP (~ 63 kD).)
NCBI and Uniprot Product Information
NCBI Description
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq, Jul 2008]
Uniprot Description
MMP14: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Up-regulated by NANOS1. Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. Belongs to the peptidase M10A family.
Protein type: Protease; EC 3.4.24.80; Motility/polarity/chemotaxis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: extracellular matrix; focal adhesion; integral to plasma membrane; Golgi lumen; cytoplasm; melanosome; plasma membrane
Molecular Function: integrin binding; protein binding; protease activator activity; zinc ion binding; metalloendopeptidase activity; calcium ion binding; transcription factor activity
Biological Process: extracellular matrix organization and biogenesis; tissue remodeling; male gonad development; negative regulation of focal adhesion formation; embryonic cranial skeleton morphogenesis; proteolysis; positive regulation of cell growth; collagen catabolic process; extracellular matrix disassembly; branching morphogenesis of a tube; regulation of transcription, DNA-dependent; ovarian follicle development; response to mechanical stimulus; response to estrogen stimulus; endothelial cell proliferation; response to hypoxia; zymogen activation; angiogenesis; response to oxidative stress; astrocyte cell migration; lung development; endochondral ossification; positive regulation of cell migration
Disease: Winchester Syndrome
Research Articles on MMP-14
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Product Notes
The MMP-14 mmp14 (Catalog #AAA6487104) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Matrix Metalloproteinase 14, Catalytic Domain, active (MT1-MMP, MMP-14, Collagenase-3) Recombinant (MaxLight 750) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Matrix Metalloproteinase 14 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). IHC: 1:500 WB: 1ug/ml IP: 10ug Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MMP-14 mmp14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Matrix Metalloproteinase 14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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