Rabbit MARK1/2/3/4 Polyclonal Antibody | anti-MARK1/2/3/4 antibody
MARK1/2/3/4 (Phospho Thr215) Polyclonal Antibody
Gene Names
MARK1; MARK; Par1c; Par-1c
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
MARK1/2/3/4; Polyclonal Antibody; MARK1/2/3/4 (Phospho Thr215) Polyclonal Antibody; MARK1; KIAA1477; MARK; Serine/threonine-protein kinase MARK1; MAP/microtubule affinity-regulating kinase 1; PAR1 homolog c; Par-1c; Par1c; MARK2; EMK1; Serine/threonine-protein kinase MARK2; ELKL motif kinase 1; EMK-1; MAP/microtubule affin; anti-MARK1/2/3/4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Phospho-MARK1/2/3/4 (T215) Polyclonal Antibody detects endogenous levels of MARK1/2/3/4 protein only when phosphorylated at T215.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MARK1/2/3/4 antibody
Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin, section), ELISA (EIA)
Application Notes
IHC: 1/100-1/300
ELISA: 1/5000
ELISA: 1/5000
Immunogen
The antiserum was produced against synthesized peptide derived from human MARK1/2/3/4 around the phosphorylation site of Thr215. AA range:181-230
Catalytic Activity
ATP + a protein = ADP + a phosphoprotein
Cofactor
Magnesium
Enzyme regulation
Activated by phosphorylation on Thr-215 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39.
Subcellular Location
Appears to localize to an intracellular network.
Tissue specificity
Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.
Similarity
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily. Contains 1 KA1 (kinase-associated) domain, Contains 1 protein kinase domain, Contains 1 UBA domain.
Preparation and Storage
Store at -20 degree C for 1 year
Related Product Information for anti-MARK1/2/3/4 antibody
Function: May play a role in cytoskeletal stability.
Product Categories/Family for anti-MARK1/2/3/4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,911 Da
NCBI Official Full Name
serine/threonine-protein kinase MARK1 isoform 2
NCBI Official Synonym Full Names
MAP/microtubule affinity-regulating kinase 1
NCBI Official Symbol
MARK1
NCBI Official Synonym Symbols
MARK; Par1c; Par-1c
NCBI Protein Information
serine/threonine-protein kinase MARK1; PAR1 homolog c
UniProt Protein Name
Serine/threonine-protein kinase MARK1
UniProt Gene Name
MARK1
UniProt Synonym Gene Names
Par-1c; Par1c
UniProt Entry Name
MARK1_HUMAN
Uniprot Description
MARK1: a member of the CAMKL family of protein kinases. The MARK group of kinases is apparently involved in the control of cell polarity, microtubule stability and cancer. MARK1 forms a tight association with Tau in neurofibrillary tangles in Alzheimer disease tissue.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.26; EC 2.7.11.1; Protein kinase, CAMK; CAMK group; CAMKL family; MARK subfamily
Chromosomal Location of Human Ortholog: 1q41
Cellular Component: microtubule cytoskeleton; cytoskeleton; cytoplasm; plasma membrane
Molecular Function: protein serine/threonine kinase activity; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylserine binding; tau-protein kinase activity; magnesium ion binding; ATP binding
Biological Process: Wnt receptor signaling pathway; neuron migration; cytoskeleton organization and biogenesis; protein amino acid phosphorylation
Research Articles on MARK1/2/3/4
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Product Notes
The MARK1/2/3/4 mark1 (Catalog #AAA9458901) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARK1/2/3/4 (Phospho Thr215) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MARK1/2/3/4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)(paraffin, section), ELISA (EIA). IHC: 1/100-1/300 ELISA: 1/5000. Researchers should empirically determine the suitability of the MARK1/2/3/4 mark1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARK1/2/3/4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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