Goat MARCH-VII Polyclonal Antibody | anti-MARCH7 antibody

MARCH-VII (Axotrophin, MARCH7, membrane-associated ring finger (C3HC4) 7, AXO, AXOT, DKFZP586F1122, MARCH-VII, RNF177)

Cat. Num. AAA619174

• Gene Names: MARCH7; AXO; AXOT; RNF177; MARCH-VII; DKFZp586F1122
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: MARCH-VII; Polyclonal Antibody; MARCH-VII (Axotrophin; MARCH7; membrane-associated ring finger (C3HC4) 7; AXO; AXOT; DKFZP586F1122; RNF177); Anti -MARCH-VII (Axotrophin; anti-MARCH7 antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human MARCH-VII. Species Crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-MARCH7 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.; Dilution: Peptide ELISA: 1:4,000.; Western Blot: Preliminary experiments gave an approx 125kD band in Human Spleen and Tonsil lysates after 1ug/ml antibody staining. Optimal dilutions to be determined by the researcher.
• Immunogen: A synthetic peptide from the C-terminus of aa--NFNQESEGRNT
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-MARCH7 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 13097303
• NCBI GeneID: 64844
• UniProt Accession #: Q9H992; Q53GQ1; Q9BTR9; A8K9X1; D3DPB0
• Molecular Weight: 78,051 Da
• NCBI Official Full Name: MARCH7 protein
• NCBI Official Synonym Full Names: membrane-associated ring finger (C3HC4) 7
• NCBI Official Symbol: MARCH7
• NCBI Official Synonym Symbols: AXO; AXOT; RNF177; MARCH-VII; DKFZp586F1122
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH7; axotrophin; OTTHUMP00000162900; OTTHUMP00000204988; OTTHUMP00000204989; RING finger protein 177; membrane-associated RING-CH protein VII; membrane-associated RING finger protein 7
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH7
• UniProt Gene Name: MARCH7
• UniProt Synonym Gene Names: AXOT; RNF177
• UniProt Entry Name: MARH7_HUMAN

NCBI Description

MARCH7 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM]

Uniprot Description

MARCH7: E3 ubiquitin-protein ligase which may specifically enhance the E2 activity of HIP2. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-; Ligase

Chromosomal Location of Human Ortholog: 2q24.2

Molecular Function: zinc ion binding; ligase activity

Biological Process: protein ubiquitination