Goat MARCH-IV Polyclonal Antibody | anti-MARCH4 antibody

Goat anti-MARCH-IV Antibody

Cat. Num. AAA421583

• Gene Names: MARCH4; RNF174; MARCH-IV
• Reactivity: Expected from sequence similarity: Human
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: MARCH-IV; Polyclonal Antibody; Goat anti-MARCH-IV Antibody; MARCH4; membrane-associated ring finger (C3HC4) 4; MGC104908; RNF174; membrane-associated RING-CH protein IV; MARCH4 antibody; membrane-associated ring finger (C3HC4) 4 antibody; MARCH-IV antibody; MGC104908 antibody; RNF174 antibody; membrane-associated RING-CH protein IV antibody; anti-MARCH4 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: DFCKEKTEDRYS
• Sequence Length: 410

• Applicable Applications for anti-MARCH4 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 4000.; Western Blot: Preliminary experiments in Human Brain (Amygdala, Cerebellum, Cerebral Cortex, Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
• Immunogen: Peptide with sequence DFCKEKTEDRYS, from the internal region of the protein sequence according to NP_065865.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 57977325
• NCBI GeneID: 57574
• NCBI Accession #: NP_065865.1
• NCBI GenBank Nucleotide #: NM_020814.2
• Molecular Weight: 45,528 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase MARCH4
• NCBI Official Synonym Full Names: membrane associated ring-CH-type finger 4
• NCBI Official Symbol: MARCH4
• NCBI Official Synonym Symbols: RNF174; MARCH-IV
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH4
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH4
• UniProt Gene Name: MARCH4
• UniProt Synonym Gene Names: KIAA1399; RNF174; MARCH-IV
• UniProt Entry Name: MARH4_HUMAN

NCBI Description

MARCH4 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH4 reduces surface accumulation of several membrane glycoproteins by directing them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]

Uniprot Description

MARCH4: E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Protein type: Membrane protein, multi-pass; Ubiquitin ligase; Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Membrane protein, integral; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: Golgi membrane; Golgi stack; integral to membrane; trans-Golgi network

Molecular Function: ligase activity; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: protein ubiquitination