Goat MARCH-I Polyclonal Antibody | anti-MARCH1 antibody

MARCH-I (Membrane-associated ring finger (C3HC4) 1, DKFZp564M1682, FLJ20668, RNF171)

Cat. Num. AAA620907

• Gene Names: MARCH1; RNF171; MARCH-I
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: MARCH-I; Polyclonal Antibody; MARCH-I (Membrane-associated ring finger (C3HC4) 1; DKFZp564M1682; FLJ20668; RNF171); Anti -MARCH-I (Membrane-associated ring finger (C3HC4) 1; anti-MARCH1 antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human MARCH-I. Species Crossreactivity: mouse, rat.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-MARCH1 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA.; Dilution: Peptide ELISA: 1:32,000.; Western Blot: Preliminary experiments in Human Colon, Ileum, Lung and Lymph Node lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). Optimal dilutions to be determined by the researcher.
• Immunogen: A synthetic peptide from the C-terminus of aa-QSRLSVCPSTQD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-MARCH1 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 157170356
• NCBI GeneID: 55016
• UniProt Accession #: Q8TCQ1; Q9NWR0; D3DP29
• Molecular Weight: 32,308 Da
• NCBI Official Full Name: Membrane-associated ring finger (C3HC4) 1
• NCBI Official Synonym Full Names: membrane-associated ring finger (C3HC4) 1, E3 ubiquitin protein ligase
• NCBI Official Symbol: MARCH1
• NCBI Official Synonym Symbols: RNF171; MARCH-I
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH1; RING finger protein 171; membrane-associated RING-CH protein I; membrane-associated RING finger protein 1
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH1
• UniProt Gene Name: MARCH1
• UniProt Synonym Gene Names: RNF171; MARCH-I
• UniProt Entry Name: MARH1_HUMAN

NCBI Description

MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).[supplied by OMIM, Mar 2010]

Uniprot Description

MARCH1: E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Ligase; Membrane protein, multi-pass; Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 4q32.2

Cellular Component: endoplasmic reticulum membrane; cytoplasmic vesicle membrane; lysosomal membrane; lysosome; late endosome membrane; early endosome membrane; integral to membrane; plasma membrane; trans-Golgi network membrane; endosome

Molecular Function: zinc ion binding; ubiquitin-protein ligase activity; MHC protein binding; ligase activity

Biological Process: protein polyubiquitination; antigen processing and presentation of peptide antigen via MHC class II; immune response