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Western Blot (WB) ((0.1ug/ml) staining of Human Brain (Amygdala) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat MAPRE3 Polyclonal Antibody | anti-MAPRE3 antibody

Goat anti-MAPRE3 Antibody

Gene Names
MAPRE3; EB3; RP3; EBF3; EBF3-S
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Dog, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
MAPRE3; Polyclonal Antibody; Goat anti-MAPRE3 Antibody; Goat Anti-MAPRE3 (aa151-164) Antibody; microtubule-associated protein; RP/EB family; member 3; EB3; EBF3; EBF3-S; RP3; APC binding protein; APC binding protein antibody; EB3 antibody; EBF3 antibody; EBF3-S antibody; member 3 antibody; RP3 antibody; MAPRE3 antibody; anti-MAPRE3 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SGRLSNVAPPCILR
Sequence Length
281
Applicable Applications for anti-MAPRE3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 32kDa band observed in Human Brain (Cerebellum and Amygdala) and Mouse Brain lysates (calculated MW of 32kDa according to NP_036458.2). Recommended concentration: 0.1-1. 0 ug/ml.
Immunogen
Peptide with sequence SGRLSNVAPPCILR, from the internal region of the protein sequence according to NP_036458.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of Human Brain (Amygdala) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.1ug/ml) staining of Human Brain (Amygdala) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,380 Da
NCBI Official Full Name
microtubule-associated protein RP/EB family member 3
NCBI Official Synonym Full Names
microtubule associated protein RP/EB family member 3
NCBI Official Symbol
MAPRE3
NCBI Official Synonym Symbols
EB3; RP3; EBF3; EBF3-S
NCBI Protein Information
microtubule-associated protein RP/EB family member 3
UniProt Protein Name
Microtubule-associated protein RP/EB family member 3
UniProt Gene Name
MAPRE3
UniProt Synonym Gene Names
EBF3; EB3
UniProt Entry Name
MARE3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RP/EB family of genes. The protein localizes to the cytoplasmic microtubule network and binds APCL, a homolog of the adenomatous polyposis coli tumor suppressor gene. [provided by RefSeq, Jul 2008]

Uniprot Description

EB3: Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. Belongs to the MAPRE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding

Chromosomal Location of Human Ortholog: 2p23.3-p23.1

Cellular Component: cytoplasm; cytoplasmic microtubule; microtubule cytoskeleton; midbody; perinuclear region of cytoplasm

Molecular Function: microtubule binding; protein binding

Biological Process: cell division; mitosis; positive regulation of cyclin-dependent protein kinase activity; positive regulation of transcription, DNA-dependent

Research Articles on MAPRE3

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Product Notes

The MAPRE3 mapre3 (Catalog #AAA421969) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MAPRE3 Antibody reacts with Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MAPRE3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 32kDa band observed in Human Brain (Cerebellum and Amygdala) and Mouse Brain lysates (calculated MW of 32kDa according to NP_036458.2). Recommended concentration: 0.1-1. 0 ug/ml. Researchers should empirically determine the suitability of the MAPRE3 mapre3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SGRLSNVAPP CILR. It is sometimes possible for the material contained within the vial of "MAPRE3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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