Rabbit anti-Human, Mouse MAPKAPK5 Polyclonal Antibody | anti-MAPKAPK5 antibody

MAPKAPK5 Antibody

Cat. Num. AAA9505329

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: MAPKAPK5; Polyclonal Antibody; MAPKAPK5 Antibody; MAPK-activated protein kinase 5; MAPKAP kinase 5; p38-regulated/activated protein kinase; anti-MAPKAPK5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: MAPKAPK5 Antibody detects endogenous levels of total MAPKAPK5 protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 471

• Applicable Applications for anti-MAPKAPK5 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50~1:100; ELISA: 1:5000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human MAPKAPK5.
• Immunogen Range: 171-220
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MAPKAPK5 Antibody. The picture on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 21237765
• NCBI GeneID: 8550
• NCBI Accession #: NP_003659.2
• NCBI GenBank Nucleotide #: NM_003668.3
• UniProt Accession #: Q8IW41; O60491; Q86X46; Q9BVX9; Q9UG86; B3KVA5
• Molecular Weight: 54 kDa
• NCBI Official Full Name: MAP kinase-activated protein kinase 5 isoform 1
• UniProt Protein Name: MAP kinase-activated protein kinase 5
• Protein Family: MAP kinase-activated protein kinase
• UniProt Gene Name: MAPKAPK5
• UniProt Synonym Gene Names: PRAK; MAPK-activated protein kinase 5; MAPKAP kinase 5; MAPKAP-K5; MAPKAPK-5; MK-5; MK5; PRAK

Uniprot Description

Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.