Western Blot (WB)
(HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with (0.5ug/ml) in the left panel and with (0.5ug/ml) in the right panel.)
Goat MAPKAPK2 Polyclonal Antibody | anti-MAPKAPK2 antibody
Goat anti-MAPKAPK2 (aa44-55) Antibody
Western Blot (WB)
(HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with (0.5ug/ml) in the left panel and with (0.5ug/ml) in the right panel.)
Western Blot (WB)
(HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with (0.5ug/ml) in the left panel and with (0.5ug/ml) in the right panel.)
Western Blot (WB)
(And (1.5ug) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp. ) were analyzed in parallel in this Western blot labelled with rabbit anti-MK2.)
Western Blot (WB)
(And (1.5ug) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp. ) were analyzed in parallel in this Western blot labelled with rabbit anti-MK2.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
MAPKAPK2: a member of the MAPKAPK family of protein kinases. It is phosphorylated and activated by p38 in response to cytokines, stress and chemotactic factors.MAPKAPK-2 is apparently a direct target of p38 MAPK. HSP27 is one of its in vivo substrates. Two transcript variants encoding two different isoforms have been described.
Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; CAMK group; MAPKAPK family; MAPKAPK subfamily
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: centrosome; cytoplasm; cytosol; nucleoplasm; nucleus
Molecular Function: ATP binding; calcium-dependent protein serine/threonine kinase activity; calmodulin binding; calmodulin-dependent protein kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; signal transducer activity
Biological Process: activation of MAPK activity; G2/M transition DNA damage checkpoint; inflammatory response; inner ear development; leukotriene metabolic process; MAPKKK cascade; peptidyl-serine phosphorylation; positive regulation of tumor necrosis factor biosynthetic process; protein amino acid autophosphorylation; protein amino acid phosphorylation; Ras protein signal transduction; regulation of interleukin-6 production; regulation of mRNA stability; regulation of tumor necrosis factor production; response to cytokine stimulus; response to DNA damage stimulus; response to lipopolysaccharide; response to stress; toll-like receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway
Research Articles on MAPKAPK2
Similar Products
Product Notes
The MAPKAPK2 mapkapk2 (Catalog #AAA422918) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-MAPKAPK2 (aa44-55) Antibody reacts with Tested: Moiuse; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAPKAPK2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB-Trf), Immunoprecipitation (IP). Peptide ELISA: Antibody detection limit dilution 1: 32000. Researchers should empirically determine the suitability of the MAPKAPK2 mapkapk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PQFHVKSGLQ IK. It is sometimes possible for the material contained within the vial of "MAPKAPK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
