Rabbit MAPKAP1 Polyclonal Antibody | anti-MAPKAP1 antibody

MAPKAP1 (Mitogen-activated Protein Kinase 2-associated Protein 1, MIP1, SIN1, JC310, SIN1b, SIN1g) (Biotin)

Cat. Num. AAA6425860

• Gene Names: MAPKAP1; MIP1; SIN1; JC310; SIN1b; SIN1g
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: MAPKAP1; Polyclonal Antibody; MAPKAP1 (Mitogen-activated Protein Kinase 2-associated Protein 1; MIP1; SIN1; JC310; SIN1b; SIN1g) (Biotin); anti-MAPKAP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes MAPKAP1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-MAPKAP1 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:100; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human MAPKAP1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of HepG2 cell line, using MAPKAP1 antibody.)

Product Categories/Family for anti-MAPKAP1 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 15214282
• NCBI GeneID: 79109
• UniProt Accession #: Q9BPZ7; Q00426; Q5JSV5; Q5JSV6; Q5JSV9; Q658R0; Q699U1; Q699U2; Q699U3; A8K1Z5; B1AMA4; B7Z309
• Molecular Weight: 522
• NCBI Official Full Name: Target of rapamycin complex 2 subunit MAPKAP1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase associated protein 1
• NCBI Official Symbol: MAPKAP1
• NCBI Official Synonym Symbols: MIP1; SIN1; JC310; SIN1b; SIN1g
• NCBI Protein Information: target of rapamycin complex 2 subunit MAPKAP1; mSIN1; TORC2 subunit MAPKAP1; ras inhibitor MGC2745; SAPK-interacting protein 1; MEKK2-interacting protein 1; stress-activated protein kinase-interacting 1; stress-activated map kinase interacting protein 1
• UniProt Protein Name: Target of rapamycin complex 2 subunit MAPKAP1
• Protein Family: Target of rapamycin complex
• UniProt Gene Name: MAPKAP1
• UniProt Synonym Gene Names: MIP1; SIN1; TORC2 subunit MAPKAP1; SAPK-interacting protein 1; mSIN1
• UniProt Entry Name: SIN1_HUMAN

NCBI Description

This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Sin1: a member of a conserved family of proteins that have an essential roles in signal transduction from yeast to mammals. An essential component of the mTORC2 complex, which consists of mTOR, LST8, Sin1, Protor (PRR5), and RICTOR, the Rapamycin Insensitive Companion of mTOR. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 regulates the phosphorylation of SGK1 S422 and PKCA S657. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Contains a Ras-binding (RBD) and a pleckstrin homology (PH) domains. Plays an important role in the SAPK signaling pathway by binding directly to both ATF-2 and p38. A genetic knockout of Sin1 abolished Akt-S473 phosphorylation and disrupts the RICTOR-mTOR interaction, but Akt-Thr308 phosphorylation is maintained. Six alternatively-spliced isoforms of the human protein have been reported. All isoforms except 4 can be incorporated into the the mTORC2 complex.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q33.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function: protein binding; phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; Ras GTPase binding; phosphatidylinositol-3,4-bisphosphate binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; substantia nigra development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; T cell costimulation; innate immune response; negative regulation of Ras protein signal transduction; vascular endothelial growth factor receptor signaling pathway