Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7004891 at 1:266,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Rabbit anti-Human, mouse MAPK9 Polyclonal Antibody | anti-MAPK9 antibody
MAPK9 Antibody
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7004891 at 1:266,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of Hela cells with MBS7004891 at 1:266,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunoprecipitation (IP)
(Immunoprecipitating MAPK9 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS7004891 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS7004891(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
Immunoprecipitation (IP)
(Immunoprecipitating MAPK9 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS7004891 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS7004891(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]
Uniprot Description
JNK2: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses including UV radiation. Phosphorylates specific transcription factors such as c-Jun and ATF2, mediating immediate-early gene expression. Closely related to JNK1. Both are involved in UV radiation induced apoptosis. Blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells in the mouse. Four alternatively-spliced isoforms have been described.
Protein type: Protein kinase, CMGC; Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; MAPK/JNK subfamily; JNK subfamily
Chromosomal Location of Human Ortholog: 5q35
Cellular Component: cytosol; mitochondrion; nucleoplasm
Molecular Function: ATP binding; caspase activator activity; JUN kinase activity; mitogen-activated protein kinase kinase kinase binding; protein binding; transcription factor binding
Biological Process: caspase activation; central nervous system development; innate immune response; JNK cascade; JUN phosphorylation; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; neurite development; positive regulation of chemokine production; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of prostaglandin biosynthetic process; positive regulation of prostaglandin secretion; positive regulation of protein amino acid phosphorylation; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; protein targeting to mitochondrion; regulation of circadian rhythm; regulation of JNK cascade; regulation of protein ubiquitination; regulation of transcription factor activity; release of cytochrome c from mitochondria; response to amine stimulus; response to cadmium ion; response to drug; response to mechanical stimulus; response to stress; response to toxin; rhythmic process; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway
Research Articles on MAPK9
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Product Notes
The MAPK9 mapk9 (Catalog #AAA7004891) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAPK9 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MAPK9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). Recommended dilution: WB:1:500-2000. Researchers should empirically determine the suitability of the MAPK9 mapk9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPK9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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