Western Blot (WB)
(MAP3K9 Antibody (N-term) western blot analysis in SW480 cell line lysates (35ug/lane).This demonstrates the MAP3K9 antibody detected the MAP3K9 protein (arrow).)
Rabbit MAP3K9 Polyclonal Antibody | anti-MAP3K9 antibody
MAP3K9 Antibody (N-term)
Gene Names
MAP3K9; MLK1; MEKK9; PRKE1
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MAP3K9; Polyclonal Antibody; MAP3K9 Antibody (N-term); Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1; MLK1; PRKE1; anti-MAP3K9 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MAP3K9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-189 amino acids from the N-terminal region of human MAP3K9.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.49 (varies by lot)
Sequence Positions
159-189
Sequence Length
1118
Applicable Applications for anti-MAP3K9 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(MAP3K9 Antibody (N-term) western blot analysis in SW480 cell line lysates (35ug/lane).This demonstrates the MAP3K9 antibody detected the MAP3K9 protein (arrow).)
Western Blot (WB)
(MAP3K9 Antibody (N-term) western blot analysis in SW480 cell line lysates (35ug/lane).This demonstrates the MAP3K9 antibody detected the MAP3K9 protein (arrow).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Related Product Information for anti-MAP3K9 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Product Categories/Family for anti-MAP3K9 antibody
References
Dorow, D.S., et al., Eur. J. Biochem. 213(2):701-710 (1993).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
121895
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 9 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 9
NCBI Official Symbol
MAP3K9
NCBI Official Synonym Symbols
MLK1; MEKK9; PRKE1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 9
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 9
Protein Family
UniProt Gene Name
MAP3K9
UniProt Synonym Gene Names
MLK1; PRKE1
UniProt Entry Name
M3K9_HUMAN
Uniprot Description
MLK1: a TKL kinase of the MLK family. A dual leucine zipper kinase. Expressed in neuronal cells and may act between Rac1/Cdc42 and MKK4 and -7 in death signaling.
Protein type: Protein kinase, TKL; Kinase, protein; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; MLK subfamily
Chromosomal Location of Human Ortholog: 14q24.2
Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; JUN kinase kinase kinase activity; protein homodimerization activity; protein-tyrosine kinase activity; ATP binding
Biological Process: peptidyl-tyrosine phosphorylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; activation of JNK activity; activation of JNKK activity
Research Articles on MAP3K9
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Product Notes
The MAP3K9 map3k9 (Catalog #AAA9207711) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 159-189. The MAP3K9 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's MAP3K9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the MAP3K9 map3k9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP3K9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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