Goat MAP3K7IP3 Polyclonal Antibody | anti-MAP3K7IP3 antibody

Goat anti-MAP3K7IP3 (aa 59 to 69) Antibody

Cat. Num. AAA420409

• Gene Names: TAB3; NAP1; MAP3K7IP3
• Reactivity: Expected from sequence similarity: Human, Mouse
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: MAP3K7IP3; Polyclonal Antibody; Goat anti-MAP3K7IP3 (aa 59 to 69) Antibody; Goat Anti-MAP3K7IP3 (aa 274 to 285) Antibody; mitogen-activated protein kinase kinase kinase 7 interacting protein 3; MGC45404; NAP1; TAB3; Mitogen-activated protein kinase kinase kinase 7-interacting protein 3; NF-kappa-B-activating protein 1; NFkB activating protein 1; TAK1 binding prote; MAP3K7IP3 antibody; mitogen-activated protein kinase kinase kinase 7 interacting protein 3 antibody; MGC45404 antibody; NAP1 antibody; TAB3 antibody; Mitogen-activated protein kinase kinase kinase 7-interacting protein 3 antibody; NF-kappa-B-activating protein 1 antibody; NFkB activating protein 1 antibody; TAK1 binding protein 3 antibody; MAP3K7IP3 (aa 59 to 69); anti-MAP3K7IP3 antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: HSPDDNRMNRN
• Sequence Length: 712

• Applicable Applications for anti-MAP3K7IP3 antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Western Blot: Preliminary experiments gave an approx 100kDa band in Human Kidney lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 78.7kDa according to NP_690000.2. The 100kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-HSPDDNRMNRN, from the internal region of the protein sequence according to NP_690000.2.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 98991767
• NCBI GeneID: 257397
• NCBI Accession #: NP_690000.2
• NCBI GenBank Nucleotide #: NM_152787.3
• Molecular Weight: 75,599 Da
• NCBI Official Full Name: TGF-beta-activated kinase 1 and MAP3K7-binding protein 3
• NCBI Official Synonym Full Names: TGF-beta activated kinase 1/MAP3K7 binding protein 3
• NCBI Official Symbol: TAB3
• NCBI Official Synonym Symbols: NAP1; MAP3K7IP3
• NCBI Protein Information: TGF-beta-activated kinase 1 and MAP3K7-binding protein 3
• UniProt Protein Name: TGF-beta-activated kinase 1 and MAP3K7-binding protein 3
• Protein Family: Mitogen-activated protein kinase kinase kinase 7-interacting protein
• UniProt Gene Name: TAB3
• UniProt Synonym Gene Names: MAP3K7IP3; TAB-3
• UniProt Entry Name: TAB3_HUMAN

NCBI Description

The product of this gene functions in the NF-kappaB signal transduction pathway. The encoded protein, and the similar and functionally redundant protein MAP3K7IP2/TAB2, forms a ternary complex with the protein kinase MAP3K7/TAK1 and either TRAF2 or TRAF6 in response to stimulation with the pro-inflammatory cytokines TNF or IL-1. Subsequent MAP3K7/TAK1 kinase activity triggers a signaling cascade leading to activation of the NF-kappaB transcription factor. The human genome contains a related pseudogene. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TAB3: Adapter linking MAP3K7/TAK1 and TRAF6 or TRAF2. Mediator of MAP3K7 activation, respectively in the IL1 and TNF signaling pathways. Plays a role in activation of NF-kappa-B and AP1 transcription factor. Isoform 2 may be an oncogenic factor. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xp21.2

Cellular Component: cytoplasm; cytosol; endosome membrane; nucleoplasm; plasma membrane

Molecular Function: protein binding; zinc ion binding

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; I-kappaB kinase/NF-kappaB cascade; JNK cascade; MyD88-dependent toll-like receptor signaling pathway; negative regulation of autophagy; stimulatory C-type lectin receptor signaling pathway