Rabbit MAP2K5 Polyclonal Antibody | anti-MAP2K5 antibody

MAP2K5, Phosphorylated (S311/T315) (Mitogen-activated Protein Kinase Kinase 5, Mek5) (FITC)

Cat. Num. AAA6425487

• Gene Names: Map2k5; Mek5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: MAP2K5; Polyclonal Antibody; Phosphorylated (S311/T315) (Mitogen-activated Protein Kinase Kinase 5; Mek5) (FITC); anti-MAP2K5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Map2k5, phosphorylated S311/T315. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-MAP2K5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding S311/T315 of human Map2k5.
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from Rat and Mouse brain tissue using phospho-Map2k5-S311/T315 antibody.)

Product Categories/Family for anti-MAP2K5 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 2499634
• NCBI GeneID: 29568
• UniProt Accession #: Q62862; Q62863; Q62864
• Molecular Weight: 448
• NCBI Official Full Name: Dual specificity mitogen-activated protein kinase kinase 5
• NCBI Official Synonym Full Names: mitogen activated protein kinase kinase 5
• NCBI Official Symbol: Map2k5
• NCBI Official Synonym Symbols: Mek5
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 5; MEK 5; MAPKK 5; MAPK/ERK kinase 5; MAP kinase kinase 5
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 5
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: Map2k5
• UniProt Synonym Gene Names: Mek5; Mkk5; Prkmk5; MAP kinase kinase 5; MAPKK 5; MEK 5
• UniProt Entry Name: MP2K5_RAT

NCBI Description

kinase involved in signal transduction in the cell [RGD, Feb 2006]

Uniprot Description

MEK5: a dual specificity protein kinase of the STE7 family. Activates ERK5 and is itself phosphorylated and activated by MEKK3, as well as by atypical protein kinase C isoforms. Involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced isoforms have been described.

Protein type: EC 2.7.12.2; Kinase, protein; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Cellular Component: membrane; spindle; intracellular; nucleus; cytosol

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; metal ion binding; protein-tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; negative regulation of heterotypic cell-cell adhesion; activation of MAPK activity; heart development; MAPKKK cascade; negative regulation of interleukin-8 biosynthetic process; negative regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; positive regulation of protein metabolic process; positive regulation of cell growth; inhibition of NF-kappaB transcription factor; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation