Rabbit Map2k3 Polyclonal Antibody | anti-MAP2K3 antibody

Map2k3, ID (Mkk3, Prkmk3, Mitogen-activated protein kinase kinase 3, MAP2K)

Cat. Num. AAA643930

• Gene Names: MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: Map2k3; Polyclonal Antibody; ID (Mkk3; Prkmk3; Mitogen-activated protein kinase kinase 3; MAP2K); Anti -Map2k3; anti-MAP2K3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAP2K3/MEK3. Species Crossreactivity: Mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-MAP2K3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunohistochemistry.; Dilution: ELISA: 1:5000; Western Blot: 1:500-1:1000; Immunohistochemistry (Paraffin): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human MAP2K3/MEK3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(MAP2K3/MEK3 IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced retrieval.)

Immunohistochemistry (IHC)

(MAP2K3/MEK3 IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced retrieval.)

Western Blot (WB)

(Western blot analysis of extracts from MDA-MB-435 cells, using MKK3 (Ab-189). The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-MAP2K3 antibody

MEK3, a MAP2K type protein kinase, is a member of the p38 MAP kinase signal transduction pathway which mediates stress induced cell cycle arrest, transcription activation and apoptosis. MEK3 can be activated by insulin and is required for glucose transporter expression. The protein is phosphorylated by ASK1 or TAO1, and in turn, it phosphorylates the MAPKs p38-alpha and -beta. At least three protein isoforms, resulting from alternatively spliced transcript variants of the MEK3 gene, have been identified - 318, 347 and 352 amino acids. Oncogenic transformation of primary cells has been linked to accumulation of activated MEK3 which results from expression of the RAS oncogene. Yersina pseudotuberculosis has been shown to block phosphorylation and subsequent activation of MEK3.

Product Categories/Family for anti-MAP2K3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 49456543
• NCBI GeneID: 5606
• UniProt Accession #: Q6FHG1; P46734
• Molecular Weight: 39,265 Da
• NCBI Official Full Name: MAP2K3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 3
• NCBI Official Symbol: MAP2K3
• NCBI Official Synonym Symbols: MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 3; MEK 3; MAPKK 3; SAPK kinase 2; MAPK/ERK kinase 3; MAP kinase kinase 3; stress-activated protein kinase kinase 2
• UniProt Protein Name: MAP2K3 protein
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K3
• UniProt Entry Name: Q6FHG1_HUMAN

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MKK3: a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. A deletion and two point mutants found in colon cancer cell lines. Three splice-variant isoforms have been described.

Protein type: Kinase, protein; EC 2.7.12.2; Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; membrane; cytosol

Molecular Function: MAP kinase kinase activity; protein binding; protein-tyrosine kinase activity; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of protein kinase activity; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway; regulation of cytokine biosynthetic process; cardiac muscle contraction