Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Paraffin-embedded rat brain tissue (Fig. 1) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:200 dilution of M2350-02F. Reactivity was detected using the IHC-Select Detection Kit In Fig 1, staining pattern appears in neuronal microtubule cells of the cerebral cortex (brown).)
Rabbit anti-Rat MAP2 Polyclonal Antibody | anti-MAP2 antibody
MAP2 (Microtubule Associated Protein 2, MAP-2) (MaxLight 650)
Gene Names
Map2; MAP2R; Mtap2
Reactivity
Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
MAP2; Polyclonal Antibody; MAP2 (Microtubule Associated Protein 2; MAP-2) (MaxLight 650); anti-MAP2 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes rat MAP-2.
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
Sequence Length
1825
Applicable Applications for anti-MAP2 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 1:200 detects MAP-2 in rat brain tissue.
WB: 0.1ug/ml detects MAP-2 on 10ug of rat brain microsomal tissue lysate.
Applications are based on unconjugated antibody.
WB: 0.1ug/ml detects MAP-2 on 10ug of rat brain microsomal tissue lysate.
Applications are based on unconjugated antibody.
Immunogen
Three linear peptides corresponding to the N-terminal and central regions of rat MAP-2 (KLH). Species sequence homology: mouse (94%), human (72%).
Conjugate
MaxLight650
Positive Control
Rat brain microsomal tissue lysate
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Paraffin-embedded rat brain tissue (Fig. 1) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:200 dilution of M2350-02F. Reactivity was detected using the IHC-Select Detection Kit In Fig 1, staining pattern appears in neuronal microtubule cells of the cerebral cortex (brown).)
Immunohistochemistry (IHC)
(Immunohistochemistry Analysis: Paraffin-embedded rat brain tissue (Fig. 1) was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:200 dilution of M2350-02F. Reactivity was detected using the IHC-Select Detection Kit In Fig 1, staining pattern appears in neuronal microtubule cells of the cerebral cortex (brown).)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Rat brain microsomal tissue lysate was probed with M2350-02F. Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MAP-2 (~260 kDa).)
Western Blot (WB)
(Western Blotting Analysis: Representative lot data. Rat brain microsomal tissue lysate was probed with M2350-02F. Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates MAP-2 (~260 kDa).)
Product Categories/Family for anti-MAP2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
microtubule-associated protein 2
NCBI Official Synonym Full Names
microtubule-associated protein 2
NCBI Official Symbol
Map2
NCBI Official Synonym Symbols
MAP2R; Mtap2
NCBI Protein Information
microtubule-associated protein 2
UniProt Protein Name
Microtubule-associated protein 2
Protein Family
UniProt Gene Name
Map2
UniProt Synonym Gene Names
Mtap2; MAP-2
UniProt Entry Name
MTAP2_RAT
NCBI Description
regulates and organizes microtubules; may serve as a scaffolding protein for neuromodulatory activities [RGD, Feb 2006]
Uniprot Description
Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Research Articles on MAP2
Similar Products
Product Notes
The MAP2 map2 (Catalog #AAA6486481) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAP2 (Microtubule Associated Protein 2, MAP-2) (MaxLight 650) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAP2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1:200 detects MAP-2 in rat brain tissue. WB: 0.1ug/ml detects MAP-2 on 10ug of rat brain microsomal tissue lysate. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MAP2 map2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
