Guinea Pig anti-Rabbit Major Histocompatibility Complex Class II DR Alpha (MHCDRa) Polyclonal Antibody | anti-MHCDRa antibody

Cy3-Linked Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)

Cat. Num. AAA2109486

• Reactivity: Rabbit
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Major Histocompatibility Complex Class II DR Alpha (MHCDRa); Polyclonal Antibody; Cy3-Linked Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa); HLA-DRA; HLADRa; HLA Class II Histocompatibility Antigen; DR Alpha Chain; anti-MHCDRa antibody

• Host: Guinea Pig
• Reactivity: Rabbit
• Clonality: Polyclonal
• Specificity: The antibody is a cavia polyclonal antibody raised against MHCDRa. It has been selected for its ability to recognize MHCDRa in immunohistochemical staining and western blotting.
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Liquid
• Concentration: >=100ug/ml (varies by lot)
• Sequence: Ala26~Cys221
• Sequence Length: 255

• Applicable Applications for anti-MHCDRa antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Conjugation: Cy3
• Cross Reactivity: Rabbit
• Preparation and Storage: Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.; Avoid repeated freeze/thaw cycles.; ; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 283837808
• NCBI GeneID: 100328929
• NCBI Accession #: NP_001164589.1
• NCBI GenBank Nucleotide #: NM_001171118.1
• UniProt Accession #: Q30847
• NCBI Official Full Name: major histocompatibility complex, class II, DR alpha
• NCBI Official Symbol: RLA-DR-ALPHA
• NCBI Protein Information: major histocompatibility complex, class II, DR alpha
• UniProt Protein Name: Integral membrane protein
• UniProt Gene Name: RLA-DR-alpha

Uniprot Description

HLA-DRB5: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; extracellular space; membrane; late endosome membrane; lysosomal membrane; plasma membrane; trans-Golgi network membrane; MHC class II protein complex; external side of plasma membrane

Molecular Function: peptide antigen binding

Biological Process: humoral immune response mediated by circulating immunoglobulin; T-helper 1 type immune response; negative regulation of T cell proliferation; inflammatory response to antigenic stimulus; detection of bacterium; regulation of interleukin-4 production; cytokine and chemokine mediated signaling pathway; negative regulation of interferon-gamma production; T cell costimulation; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; immunoglobulin production during immune response; T cell receptor signaling pathway; protein tetramerization